Virus isolation from the first patient with SARS-CoV-2 in Korea

Wan Beom Park; Nak Jung Kwon; Su Jin Choi; Chang Kyung Kang; Pyoeng Gyun Choe; Jin Yong Kim; Jiyoung Yun; Gir Won Lee; Moon Woo Seong; Nam Joong Kim; Jeong Sun Seo; Myoung Don Oh

doi:10.3346/jkms.2020.35.e84

Virus isolation from the first patient with SARS-CoV-2 in Korea

Wan Beom Park, Nak Jung Kwon, Su Jin Choi, Chang Kyung Kang, Pyoeng Gyun Choe, Jin Yong Kim, Jiyoung Yun, Gir Won Lee, Moon Woo Seong, Nam Joong Kim, Jeong Sun Seo, Myoung Don Oh

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169 Scopus citations

Abstract

Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.

Original language	English
Article number	e84
Journal	Journal of Korean Medical Science
Volume	35
Issue number	7
DOIs	https://doi.org/10.3346/jkms.2020.35.e84
State	Published - 24 Feb 2020

Bibliographical note

Publisher Copyright:
© 2020 The Korean Academy of Medical Sciences.

Keywords

2019 Novel Coronavirus
Culture
Korea
Microscopy, Electron
Phylogeny

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10.3346/jkms.2020.35.e84

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title = "Virus isolation from the first patient with SARS-CoV-2 in Korea",

abstract = "Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.",

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doi = "10.3346/jkms.2020.35.e84",

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AU - Park, Wan Beom

AU - Kwon, Nak Jung

AU - Choi, Su Jin

AU - Kang, Chang Kyung

AU - Choe, Pyoeng Gyun

AU - Kim, Jin Yong

AU - Yun, Jiyoung

AU - Lee, Gir Won

AU - Seong, Moon Woo

AU - Kim, Nam Joong

AU - Seo, Jeong Sun

AU - Oh, Myoung Don

PY - 2020/2/24

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AB - Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.

KW - 2019 Novel Coronavirus

KW - Culture

KW - Korea

KW - Microscopy, Electron

KW - Phylogeny

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JO - Journal of Korean Medical Science

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IS - 7

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ER -

Virus isolation from the first patient with SARS-CoV-2 in Korea

Abstract

Bibliographical note

Keywords

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