Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories

a Questionnaire Survey

Clinical Mass Spectrometry Research Committee of Korean Society of Clinical Chemistry

Research output: Contribution to journalArticleResearchpeer-review

Abstract

BACKGROUND: The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has substantially increased in clinical laboratories worldwide. To assess the status of clinical LC-MS/MS testing in Korean laboratories, a questionnaire survey was performed by the Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry. METHODS: The questionnaire was distributed to 19 clinical laboratories performing clinical LC-MS/MS from April to May 2018. It asked about general characteristics of the laboratory and commonly utilized clinical LC-MS/MS tests: newborn screening, tacrolimus test, vitamin D test, and plasma metanephrine test. Frequency analysis and other statistical analyses were performed. RESULTS: A total of 17 laboratories responded. The median number of LC-MS/MS instruments, laboratory medicine physicians, and technicians in each laboratory was three, one, and two, respectively. Nine laboratory directors had >10 years of experience with clinical LC-MS/MS. For each LC-MS/MS test, at least two concentrations of QC materials were measured every 24 hours during clinical testing, and all laboratories used QC acceptability criteria based on their established QC means and SDs. All laboratories participated in an external quality assessment program. However, there was inter-laboratory variability in sample preparation methods, instruments, reagents, internal standards, and calibrators. CONCLUSIONS: LC-MS/MS has been successfully introduced in Korean clinical laboratories and is used within a quality framework. Further efforts for harmonization on a nationwide basis could facilitate the widespread use of LC-MS/MS.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalAnnals of laboratory medicine
Volume39
Issue number5
DOIs
StatePublished - 1 Sep 2019

Fingerprint

Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Testing
Clinical laboratories
Metanephrine
Surveys and Questionnaires
Tacrolimus
Vitamin D
Clinical Chemistry
Medicine
Screening
Plasmas
Mass Spectrometry
Newborn Infant
Physicians

Keywords

  • Harmonization
  • Korea
  • Liquid chromatography-tandem mass spectrometry
  • Survey

Cite this

Clinical Mass Spectrometry Research Committee of Korean Society of Clinical Chemistry. / Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories : a Questionnaire Survey. In: Annals of laboratory medicine. 2019 ; Vol. 39, No. 5. pp. 447-453.
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title = "Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories: a Questionnaire Survey",
abstract = "BACKGROUND: The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has substantially increased in clinical laboratories worldwide. To assess the status of clinical LC-MS/MS testing in Korean laboratories, a questionnaire survey was performed by the Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry. METHODS: The questionnaire was distributed to 19 clinical laboratories performing clinical LC-MS/MS from April to May 2018. It asked about general characteristics of the laboratory and commonly utilized clinical LC-MS/MS tests: newborn screening, tacrolimus test, vitamin D test, and plasma metanephrine test. Frequency analysis and other statistical analyses were performed. RESULTS: A total of 17 laboratories responded. The median number of LC-MS/MS instruments, laboratory medicine physicians, and technicians in each laboratory was three, one, and two, respectively. Nine laboratory directors had >10 years of experience with clinical LC-MS/MS. For each LC-MS/MS test, at least two concentrations of QC materials were measured every 24 hours during clinical testing, and all laboratories used QC acceptability criteria based on their established QC means and SDs. All laboratories participated in an external quality assessment program. However, there was inter-laboratory variability in sample preparation methods, instruments, reagents, internal standards, and calibrators. CONCLUSIONS: LC-MS/MS has been successfully introduced in Korean clinical laboratories and is used within a quality framework. Further efforts for harmonization on a nationwide basis could facilitate the widespread use of LC-MS/MS.",
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Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories : a Questionnaire Survey. / Clinical Mass Spectrometry Research Committee of Korean Society of Clinical Chemistry.

In: Annals of laboratory medicine, Vol. 39, No. 5, 01.09.2019, p. 447-453.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories

T2 - a Questionnaire Survey

AU - Clinical Mass Spectrometry Research Committee of Korean Society of Clinical Chemistry

AU - Chae, Hyojin

AU - Cho, Sung Eun

AU - Park, Hyung Doo

AU - Chun, Sail

AU - Lee, Yong Wha

AU - Yun, Yeo Min

AU - Song, Sang Hoon

AU - Song, Sang Hoon

AU - Lee, Kyunghoon

AU - Lee, Kyung Hoon

AU - Song, Junghan

N1 - © The Korean Society for Laboratory Medicine.

PY - 2019/9/1

Y1 - 2019/9/1

N2 - BACKGROUND: The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has substantially increased in clinical laboratories worldwide. To assess the status of clinical LC-MS/MS testing in Korean laboratories, a questionnaire survey was performed by the Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry. METHODS: The questionnaire was distributed to 19 clinical laboratories performing clinical LC-MS/MS from April to May 2018. It asked about general characteristics of the laboratory and commonly utilized clinical LC-MS/MS tests: newborn screening, tacrolimus test, vitamin D test, and plasma metanephrine test. Frequency analysis and other statistical analyses were performed. RESULTS: A total of 17 laboratories responded. The median number of LC-MS/MS instruments, laboratory medicine physicians, and technicians in each laboratory was three, one, and two, respectively. Nine laboratory directors had >10 years of experience with clinical LC-MS/MS. For each LC-MS/MS test, at least two concentrations of QC materials were measured every 24 hours during clinical testing, and all laboratories used QC acceptability criteria based on their established QC means and SDs. All laboratories participated in an external quality assessment program. However, there was inter-laboratory variability in sample preparation methods, instruments, reagents, internal standards, and calibrators. CONCLUSIONS: LC-MS/MS has been successfully introduced in Korean clinical laboratories and is used within a quality framework. Further efforts for harmonization on a nationwide basis could facilitate the widespread use of LC-MS/MS.

AB - BACKGROUND: The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has substantially increased in clinical laboratories worldwide. To assess the status of clinical LC-MS/MS testing in Korean laboratories, a questionnaire survey was performed by the Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry. METHODS: The questionnaire was distributed to 19 clinical laboratories performing clinical LC-MS/MS from April to May 2018. It asked about general characteristics of the laboratory and commonly utilized clinical LC-MS/MS tests: newborn screening, tacrolimus test, vitamin D test, and plasma metanephrine test. Frequency analysis and other statistical analyses were performed. RESULTS: A total of 17 laboratories responded. The median number of LC-MS/MS instruments, laboratory medicine physicians, and technicians in each laboratory was three, one, and two, respectively. Nine laboratory directors had >10 years of experience with clinical LC-MS/MS. For each LC-MS/MS test, at least two concentrations of QC materials were measured every 24 hours during clinical testing, and all laboratories used QC acceptability criteria based on their established QC means and SDs. All laboratories participated in an external quality assessment program. However, there was inter-laboratory variability in sample preparation methods, instruments, reagents, internal standards, and calibrators. CONCLUSIONS: LC-MS/MS has been successfully introduced in Korean clinical laboratories and is used within a quality framework. Further efforts for harmonization on a nationwide basis could facilitate the widespread use of LC-MS/MS.

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KW - Korea

KW - Liquid chromatography-tandem mass spectrometry

KW - Survey

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DO - 10.3343/alm.2019.39.5.447

M3 - Article

VL - 39

SP - 447

EP - 453

JO - Annals of Laboratory Medicine

JF - Annals of Laboratory Medicine

SN - 2234-3806

IS - 5

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