Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai

Yong Seok Lee, Sun Lim Choi, Tae Hyung Kim, Jin A. Lee, Kyu Kim Hyong, Hyoung Kim, Deok Jin Jang, Jennifer J. Lee, Sunghoon Lee, Sik Sin Gwang, Chang Bae Kim, Yutaka Suzuki, Sumio Sugano, Tai Kubo, Leonid L. Moroz, Eric R. Kandel, Jong Bhak, Bong Kiun Kaang

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.

Original languageEnglish
Pages (from-to)18602-18607
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number47
DOIs
StatePublished - 25 Nov 2008

Keywords

  • Expressed sequence tag
  • Long-term facilitation

Cite this