Transcription factor ZFHX3 regulates calcium influx in mammary epithelial cells in part via the TRPV6 calcium channel

Dan Zhao, Xueying Han, Lili Huang, Jianpeng Wang, Xi Zhang, Ju Hong Jeon, Qiang Zhao, Jin Tang Dong

Research output: Contribution to journalArticle

Abstract

Zinc finger homeobox 3 (ZFHX3) is a transcription factor that regulates multiple cellular processes including cell proliferation, differentiation and neoplastic development. It is also involved in the function of steroid hormones estrogen and progesterone and the peptide hormone prolactin in mammary epithelial cells. In this study, we investigated whether and how ZFHX3 regulates intracellular calcium homeostasis in mammary epithelial cells. We found that ZFHX3 affected both store operated calcium entry and store independent calcium entry (SOCE and SICE). Simultaneously, the expression of the calcium channel TRPV6 was regulated by ZFHX3, as demonstrated by expression analysis and luciferase reporter assay. In cells with knockdown of ZFHX3, calcium entry was partially rescued by the overexpression of wild type but not the pore mutants of TRPV6. In addition, overexpression of TRPV6 promoted differentiation of the MCF10A mammary epithelial cells in three-dimensional culture, which is consistent with our previous findings that ZFHX3 is essential for mammary gland differentiation. These findings suggest that ZFHX3 plays an important role in intracellular calcium homeostasis in mammary epithelial cells, at least in part, by regulating TRPV6.

Original languageEnglish
Pages (from-to)366-371
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume519
Issue number2
DOIs
StatePublished - 5 Nov 2019

Fingerprint

Homeobox Genes
Zinc Fingers
Calcium Channels
Zinc
Breast
Transcription Factors
Epithelial Cells
Calcium
Homeostasis
Steroid hormones
Peptide Hormones
Cell proliferation
Human Mammary Glands
TRPV6 channel
Luciferases
Prolactin
Progesterone
Cell Differentiation
Assays
Estrogens

Keywords

  • Calcium
  • Mammary epithelial cell differentiation
  • MCF10A
  • TRPV6
  • ZFHX3

Cite this

Zhao, Dan ; Han, Xueying ; Huang, Lili ; Wang, Jianpeng ; Zhang, Xi ; Jeon, Ju Hong ; Zhao, Qiang ; Dong, Jin Tang. / Transcription factor ZFHX3 regulates calcium influx in mammary epithelial cells in part via the TRPV6 calcium channel. In: Biochemical and Biophysical Research Communications. 2019 ; Vol. 519, No. 2. pp. 366-371.
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Transcription factor ZFHX3 regulates calcium influx in mammary epithelial cells in part via the TRPV6 calcium channel. / Zhao, Dan; Han, Xueying; Huang, Lili; Wang, Jianpeng; Zhang, Xi; Jeon, Ju Hong; Zhao, Qiang; Dong, Jin Tang.

In: Biochemical and Biophysical Research Communications, Vol. 519, No. 2, 05.11.2019, p. 366-371.

Research output: Contribution to journalArticle

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