Three functional isoforms of GAR-2, a Caenorhabditis elegans G-protein-linked acetylcholine receptor, are produced by alternative splicing

Su Jeong Suh, Yang Seo Park, Yong Seok Lee, Tae Ju Cho, Bong Kiun Kaang, Nam Jeong Cho

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We have previously isolated a cDNA clone from Caenorhabditis elegans that encodes a novel form of G-protein-linked acetylcholine receptor, termed GAR-2. GAR-2 is similar to but pharmacologically distinct from muscarinic acetylcholine receptors. Here we report the identification of two gar-2 cDNA clones that are different from the previous one. These newly identified cDNAs encode polypeptides of 664 and 627 amino acids, whereas the previous one encodes a polypeptide of 614 amino acids. The three GAR-2 isoforms, which differ only in the third intracellular loop, arise from alternative splicing. Electrophysiological analyses using the Xenopus oocyte system showed that all three GAR-2 isoforms couple to the activation of G-protein-gated inwardly rectifying K+ (GIRK1) channel with similar drug specificity. Our results indicate that alternative splicing plays an important role in promoting molecular diversity of G-protein-linked acetylcholine receptors in C. elegans.

Original languageEnglish
Pages (from-to)1238-1243
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number5
StatePublished - 16 Nov 2001


  • Alternative splicing
  • Caenorhabditis elegans
  • G-protein-linked acetylcholine receptor
  • Gar-2

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