The transient receptor potential protein homologue TRPC4/5 as a candidate for the nonselective cationic channel activated by muscarinic stimulation in the murine stomach

Mei Hong Zhu, Young Mee Lee, Nange Jin, Insuk So, Ki Whan Kim

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12 Citations (Scopus)

Abstract

The transient receptor potential protein homologue TRPC5 was reported as a molecular identity for the muscarinic receptor-activated nonselective cationic channel (NSCC) in the murine stomach smooth muscle. The canonical, or classical, transient receptor potential proteins, TRPC4 and TRPC5, were suggested as members of the same subfamily of TRPC channels and to be coexpressed as a heteromultimer of both TRPC as well as a homotetramer of each TRPC protein. Thus, we investigated whether the TRPC4 channel is also responsible for the NSCC activated by acetylcholine (ACh) or carbachol (CCh) using electrophysiological techniques. The TRPC channels were expressed in HEK293 cells. When murine TRPC4 channels (mTRPC4) were expressed, the current-voltage relationship of mTRPC4 was also similar to that recorded in native murine gastric myocytes or mTRPCS-expressing HEK cells. With 0.2 mM GTPγS in the pipette solution, the currents in mTRPC4-expressing cells were activated transiently like those in NSCC in the murine stomach and the expressed mTRPCS. The currents recorded in mTRPC4-expressing cells were inhibited by 1 mM La3+ and 100 μM flufenamate. The currents recorded in mTRPC4-expressing cells depended on the extracellular calcium concentration. From the above results, we suggest that mTRPC4/5 might be candidates for the NSCC activated by ACh or CCh in the murine stomach.

Original languageEnglish
Pages (from-to)302-307
Number of pages6
JournalNeurophysiology
Volume35
Issue number3-4
DOIs
StatePublished - 1 May 2003

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Cholinergic Agents
Stomach
Carbachol
Acetylcholine
Proteins
HEK293 Cells
Muscarinic Receptors
Muscle Cells
Smooth Muscle
Calcium

Keywords

  • Canonical transient receptor potential protein (TRPC)
  • Murine stomach
  • Muscarinic stimulation
  • Nonselective cation channel

Cite this

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title = "The transient receptor potential protein homologue TRPC4/5 as a candidate for the nonselective cationic channel activated by muscarinic stimulation in the murine stomach",
abstract = "The transient receptor potential protein homologue TRPC5 was reported as a molecular identity for the muscarinic receptor-activated nonselective cationic channel (NSCC) in the murine stomach smooth muscle. The canonical, or classical, transient receptor potential proteins, TRPC4 and TRPC5, were suggested as members of the same subfamily of TRPC channels and to be coexpressed as a heteromultimer of both TRPC as well as a homotetramer of each TRPC protein. Thus, we investigated whether the TRPC4 channel is also responsible for the NSCC activated by acetylcholine (ACh) or carbachol (CCh) using electrophysiological techniques. The TRPC channels were expressed in HEK293 cells. When murine TRPC4 channels (mTRPC4) were expressed, the current-voltage relationship of mTRPC4 was also similar to that recorded in native murine gastric myocytes or mTRPCS-expressing HEK cells. With 0.2 mM GTPγS in the pipette solution, the currents in mTRPC4-expressing cells were activated transiently like those in NSCC in the murine stomach and the expressed mTRPCS. The currents recorded in mTRPC4-expressing cells were inhibited by 1 mM La3+ and 100 μM flufenamate. The currents recorded in mTRPC4-expressing cells depended on the extracellular calcium concentration. From the above results, we suggest that mTRPC4/5 might be candidates for the NSCC activated by ACh or CCh in the murine stomach.",
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The transient receptor potential protein homologue TRPC4/5 as a candidate for the nonselective cationic channel activated by muscarinic stimulation in the murine stomach. / Zhu, Mei Hong; Lee, Young Mee; Jin, Nange; So, Insuk; Kim, Ki Whan.

In: Neurophysiology, Vol. 35, No. 3-4, 01.05.2003, p. 302-307.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Kim, Ki Whan

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