The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress

Jee In Heo, Mi Suk Lee, Jeong Hyun Kim, Jae Seon Lee, Jaebong Kim, Jae Bong Park, Jae Yong Lee, Jeong A. Han, Jong-Il Kim

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

The inducible 70 kDa heat shock proteins (Hsp70) in mice are encoded by two almost identical genes, hsp70.1 and hsp70.3. Studies have found that only hsp70.1 is induced by hypertonic stress while both hsp70.1 and hsp70.3 genes are expressed in response to heat shock stress. It is unclear if the human counterparts, hsp70-2 and hsp70-1, are differentially regulated by heat shock and osmotic stress. This study found that only hsp70-2 was induced by hypertonic stress in human embryonic kidney epithelial cells and fibroblasts, while heat shock stress induced both hsp70-1 and hsp70-2. The human hsp70-2 promoter region contains three TonE (tonicity-responsive enhancer) sites, which were reported to play an important role in the response to hypertonicity. When the reporter plasmids containing different parts of the 5′ flanking region of hsp70-2 were transfected into human embryonic kidney epithelial cells or fibroblasts, one TonE site at -135 was found to play a key role in the response to hypertonicity. The inactivation of the TonE site using site-directed mutagenesis led to the complete loss of induction by hypertonicity, which demonstrates the essential role of the TonE site. This suggests that the TonE site and the TonEBP (TonE binding protein) are the major regulators for the cellular response against high osmolarity in human kidney tissue.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalExperimental and Molecular Medicine
Volume38
Issue number3
DOIs
StatePublished - 30 Jun 2006

Fingerprint

Osmotic Pressure
Fibroblasts
Genes
NFATC Transcription Factors
Mutagenesis
HSP70 Heat-Shock Proteins
Kidney
5' Flanking Region
Shock
Genetic Promoter Regions
Hot Temperature
Epithelial Cells
Plasmids
Heat-Shock Response
Tissue
Site-Directed Mutagenesis
Osmolar Concentration
heat-shock protein 70.1

Keywords

  • Gene expression regulation
  • Heat-shock proteins
  • HSP70 heat-shock proteins
  • Stress
  • Transcription factors

Cite this

Heo, Jee In ; Lee, Mi Suk ; Kim, Jeong Hyun ; Lee, Jae Seon ; Kim, Jaebong ; Park, Jae Bong ; Lee, Jae Yong ; Han, Jeong A. ; Kim, Jong-Il. / The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress. In: Experimental and Molecular Medicine. 2006 ; Vol. 38, No. 3. pp. 295-301.
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abstract = "The inducible 70 kDa heat shock proteins (Hsp70) in mice are encoded by two almost identical genes, hsp70.1 and hsp70.3. Studies have found that only hsp70.1 is induced by hypertonic stress while both hsp70.1 and hsp70.3 genes are expressed in response to heat shock stress. It is unclear if the human counterparts, hsp70-2 and hsp70-1, are differentially regulated by heat shock and osmotic stress. This study found that only hsp70-2 was induced by hypertonic stress in human embryonic kidney epithelial cells and fibroblasts, while heat shock stress induced both hsp70-1 and hsp70-2. The human hsp70-2 promoter region contains three TonE (tonicity-responsive enhancer) sites, which were reported to play an important role in the response to hypertonicity. When the reporter plasmids containing different parts of the 5′ flanking region of hsp70-2 were transfected into human embryonic kidney epithelial cells or fibroblasts, one TonE site at -135 was found to play a key role in the response to hypertonicity. The inactivation of the TonE site using site-directed mutagenesis led to the complete loss of induction by hypertonicity, which demonstrates the essential role of the TonE site. This suggests that the TonE site and the TonEBP (TonE binding protein) are the major regulators for the cellular response against high osmolarity in human kidney tissue.",
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The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress. / Heo, Jee In; Lee, Mi Suk; Kim, Jeong Hyun; Lee, Jae Seon; Kim, Jaebong; Park, Jae Bong; Lee, Jae Yong; Han, Jeong A.; Kim, Jong-Il.

In: Experimental and Molecular Medicine, Vol. 38, No. 3, 30.06.2006, p. 295-301.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress

AU - Heo, Jee In

AU - Lee, Mi Suk

AU - Kim, Jeong Hyun

AU - Lee, Jae Seon

AU - Kim, Jaebong

AU - Park, Jae Bong

AU - Lee, Jae Yong

AU - Han, Jeong A.

AU - Kim, Jong-Il

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AB - The inducible 70 kDa heat shock proteins (Hsp70) in mice are encoded by two almost identical genes, hsp70.1 and hsp70.3. Studies have found that only hsp70.1 is induced by hypertonic stress while both hsp70.1 and hsp70.3 genes are expressed in response to heat shock stress. It is unclear if the human counterparts, hsp70-2 and hsp70-1, are differentially regulated by heat shock and osmotic stress. This study found that only hsp70-2 was induced by hypertonic stress in human embryonic kidney epithelial cells and fibroblasts, while heat shock stress induced both hsp70-1 and hsp70-2. The human hsp70-2 promoter region contains three TonE (tonicity-responsive enhancer) sites, which were reported to play an important role in the response to hypertonicity. When the reporter plasmids containing different parts of the 5′ flanking region of hsp70-2 were transfected into human embryonic kidney epithelial cells or fibroblasts, one TonE site at -135 was found to play a key role in the response to hypertonicity. The inactivation of the TonE site using site-directed mutagenesis led to the complete loss of induction by hypertonicity, which demonstrates the essential role of the TonE site. This suggests that the TonE site and the TonEBP (TonE binding protein) are the major regulators for the cellular response against high osmolarity in human kidney tissue.

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DO - 10.1038/emm.2006.35

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