The role of histone chaperones in osteoblastic differentiation of C2C12 myoblasts

Tae Yang Song, Jae Hyun Yang, Jin Young Park, Yunkyoung Song, Jeung Whan Han, Hong-Duk Youn, Eun Jung Cho

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Cellular differentiation is a process in which the cells gain a more specialized shape, metabolism, and function. These cellular changes are accompanied by dynamic changes in gene expression programs. In most cases, DNA methylation, histone modification, and variant histones drive the epigenetic transition that reprograms the gene expression. Histone chaperones, HIRA and Asf1a, have a role for cellular differentiation by deposition of one of variant histones, H3.3, during myogenesis of murine C2C12 cells. In this study, we accessed the roles of histone chaperones and histone H3.3 in osteoblastic conversion of C2C12 myoblasts and compared their roles with those for myogenic differentiation. The unbiased analysis of the expression pattern of histone chaperones and variant histones proposed their uncommon contribution to each pathway. HIRA and Asf1a decreased to ~50% and further diminished during differentiation into osteoblasts, while they were maintained during differentiation into myotubes. HIRA, Asf1a, and H3.3 were indispensable for expression of cell type-specific genes during conversion into osteoblasts or myotubes. RNA interference analysis indicated that histone chaperones and H3.3 were required for early steps of osteoblastic differentiation. Our results suggest that histone chaperones and variant histones might be differentially required for the distinct phases of differentiation pathway.

Original languageEnglish
Pages (from-to)726-732
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume423
Issue number4
DOIs
StatePublished - 13 Jul 2012

Fingerprint

Histone Chaperones
Myoblasts
Histones
Skeletal Muscle Fibers
Osteoblasts
Gene expression
Histone Code
Gene Expression
Gene Conversion
Muscle Development
DNA Methylation
RNA Interference
Epigenomics
Metabolism
Genes
Cells
RNA

Keywords

  • Asf1
  • Cell differentiation
  • HIRA
  • Histone chaperone
  • Myogenesis
  • Osteoblastogenesis

Cite this

Song, Tae Yang ; Yang, Jae Hyun ; Park, Jin Young ; Song, Yunkyoung ; Han, Jeung Whan ; Youn, Hong-Duk ; Cho, Eun Jung. / The role of histone chaperones in osteoblastic differentiation of C2C12 myoblasts. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 423, No. 4. pp. 726-732.
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abstract = "Cellular differentiation is a process in which the cells gain a more specialized shape, metabolism, and function. These cellular changes are accompanied by dynamic changes in gene expression programs. In most cases, DNA methylation, histone modification, and variant histones drive the epigenetic transition that reprograms the gene expression. Histone chaperones, HIRA and Asf1a, have a role for cellular differentiation by deposition of one of variant histones, H3.3, during myogenesis of murine C2C12 cells. In this study, we accessed the roles of histone chaperones and histone H3.3 in osteoblastic conversion of C2C12 myoblasts and compared their roles with those for myogenic differentiation. The unbiased analysis of the expression pattern of histone chaperones and variant histones proposed their uncommon contribution to each pathway. HIRA and Asf1a decreased to ~50{\%} and further diminished during differentiation into osteoblasts, while they were maintained during differentiation into myotubes. HIRA, Asf1a, and H3.3 were indispensable for expression of cell type-specific genes during conversion into osteoblasts or myotubes. RNA interference analysis indicated that histone chaperones and H3.3 were required for early steps of osteoblastic differentiation. Our results suggest that histone chaperones and variant histones might be differentially required for the distinct phases of differentiation pathway.",
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The role of histone chaperones in osteoblastic differentiation of C2C12 myoblasts. / Song, Tae Yang; Yang, Jae Hyun; Park, Jin Young; Song, Yunkyoung; Han, Jeung Whan; Youn, Hong-Duk; Cho, Eun Jung.

In: Biochemical and Biophysical Research Communications, Vol. 423, No. 4, 13.07.2012, p. 726-732.

Research output: Contribution to journalArticleResearchpeer-review

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