The mechanism of interferon-γ induced cytotoxicity on the lung cancer cell line, A549

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Abstract

Background: Interferon-γ has various biologic effects, including antiviral effect, antitumor proliferative effect, activation of macrophage and B lymphocyte, and increased expression of major histocompatibility complex. Especially, antitumor proliferative effect of interferon-γ has already been proved to be important in vivo as well as in vitro. And, clinical studies of interferon-γ have been tried in lung cancer patients. However, the mechanism of antitumor effect of interferon-γ has not yet been established despite many hypotheses. 'Necrosis' is a type of cell death which is well known to occur in the circumstances of severe stress. In contrast, 'apoptosis' is another type of cell death which occurs in such biological circumstances as embryonic development, regression of organs, and self-tolerance of lymphocytes. And, apoptosis is an active process of cell death in which cells are dying with fragmentations of their cytoplasms and nuclei. And, in the process of apoptosis the DNAs of cells are cleaved between nucleosomes by unidentified endonuclease and therefore DNAs of apoptotic cells result in a typical electrophoresis pattern known as DNA ladder pattern. Recently it has been suggested that cytotoxic effect of interferon-γ occurs via apoptosis. To elucidate the mechanism of antitumor cytotoxic effect of interferon-γ, we microscopically observed a lung cancer cell line, A549 which was treated with interferon-γ. We observed A545 treated with interferon-γ was dying fragmented. And so, we performed this study to find out that the mechanism of antitumor cytotoxic effect of interferon-γ be apoptosis. Method: We treated A549, human lung cancer cell line with various concentrations of interferon-γ and quantified its cytotoxic effect of various periods, 24 hours, 72 hours and, 120 hours by MTT (dimethylthiazolyl diphenyltetrazolium bromide) bioassay. Also, after we treated A549 with 100 units/ml of interferon-γ for 120 hours, we observed the pattern of cell death with inverted microscope and we extracted DNAs from the dead A549 cells and observed the pattern of 1.5% agarose gel electrophoresis with ethidium bromide staining. Result: 1) Cytotoxic effect of interferon-γ on A549: For the first 24 hours, there was little cytotoxic effect and for between 24 hours and 72 hours, there was the beginning of cytotoxic effect and for 120 hours there was increased cytotoxic effect. 2) Pattern of A549 cell death by interferon-γ: We observed with inverted microscope that A549 cells were dying fragmented. 3) DNA ladder pattern of gel electrophoresis: We observed DNA ladder pattern of gel electrophoresis of extracted DNAs from dead A549 cells. Conclusion: We concluded that the mechanism of interferon-γ induced cytotoxicity on lung cancer cell line, A549 be via apoptosis.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalTuberculosis and Respiratory Diseases
Volume43
Issue number1
DOIs
StatePublished - 1 Jan 1996

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Interferons
Lung Neoplasms
Apoptosis
Cell Death
DNA
Electrophoresis
A549 Cells
Gels
Self Tolerance
Ethidium
Macrophage Activation
Agar Gel Electrophoresis
Nucleosomes
Deoxyribonuclease I
Major Histocompatibility Complex
Bromides
Biological Assay
Embryonic Development
Antiviral Agents
Cytoplasm

Keywords

  • A549 and apoptosis
  • interferon-γ

Cite this

@article{ede3a892699f48119716b79d5b7cf286,
title = "The mechanism of interferon-γ induced cytotoxicity on the lung cancer cell line, A549",
abstract = "Background: Interferon-γ has various biologic effects, including antiviral effect, antitumor proliferative effect, activation of macrophage and B lymphocyte, and increased expression of major histocompatibility complex. Especially, antitumor proliferative effect of interferon-γ has already been proved to be important in vivo as well as in vitro. And, clinical studies of interferon-γ have been tried in lung cancer patients. However, the mechanism of antitumor effect of interferon-γ has not yet been established despite many hypotheses. 'Necrosis' is a type of cell death which is well known to occur in the circumstances of severe stress. In contrast, 'apoptosis' is another type of cell death which occurs in such biological circumstances as embryonic development, regression of organs, and self-tolerance of lymphocytes. And, apoptosis is an active process of cell death in which cells are dying with fragmentations of their cytoplasms and nuclei. And, in the process of apoptosis the DNAs of cells are cleaved between nucleosomes by unidentified endonuclease and therefore DNAs of apoptotic cells result in a typical electrophoresis pattern known as DNA ladder pattern. Recently it has been suggested that cytotoxic effect of interferon-γ occurs via apoptosis. To elucidate the mechanism of antitumor cytotoxic effect of interferon-γ, we microscopically observed a lung cancer cell line, A549 which was treated with interferon-γ. We observed A545 treated with interferon-γ was dying fragmented. And so, we performed this study to find out that the mechanism of antitumor cytotoxic effect of interferon-γ be apoptosis. Method: We treated A549, human lung cancer cell line with various concentrations of interferon-γ and quantified its cytotoxic effect of various periods, 24 hours, 72 hours and, 120 hours by MTT (dimethylthiazolyl diphenyltetrazolium bromide) bioassay. Also, after we treated A549 with 100 units/ml of interferon-γ for 120 hours, we observed the pattern of cell death with inverted microscope and we extracted DNAs from the dead A549 cells and observed the pattern of 1.5{\%} agarose gel electrophoresis with ethidium bromide staining. Result: 1) Cytotoxic effect of interferon-γ on A549: For the first 24 hours, there was little cytotoxic effect and for between 24 hours and 72 hours, there was the beginning of cytotoxic effect and for 120 hours there was increased cytotoxic effect. 2) Pattern of A549 cell death by interferon-γ: We observed with inverted microscope that A549 cells were dying fragmented. 3) DNA ladder pattern of gel electrophoresis: We observed DNA ladder pattern of gel electrophoresis of extracted DNAs from dead A549 cells. Conclusion: We concluded that the mechanism of interferon-γ induced cytotoxicity on lung cancer cell line, A549 be via apoptosis.",
keywords = "A549 and apoptosis, interferon-γ",
author = "Oh, {Y. M.} and Yoo, {Chul Gyu} and Chung, {Hee Soon} and Youngwhan Kim and Sungkoo Han and Shim, {Y. S.}",
year = "1996",
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language = "English",
volume = "43",
pages = "63--68",
journal = "Tuberculosis and Respiratory Diseases",
issn = "1738-3536",
publisher = "The Korean Academy of Tuberculosis and Respiratory Diseases",
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TY - JOUR

T1 - The mechanism of interferon-γ induced cytotoxicity on the lung cancer cell line, A549

AU - Oh, Y. M.

AU - Yoo, Chul Gyu

AU - Chung, Hee Soon

AU - Kim, Youngwhan

AU - Han, Sungkoo

AU - Shim, Y. S.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Background: Interferon-γ has various biologic effects, including antiviral effect, antitumor proliferative effect, activation of macrophage and B lymphocyte, and increased expression of major histocompatibility complex. Especially, antitumor proliferative effect of interferon-γ has already been proved to be important in vivo as well as in vitro. And, clinical studies of interferon-γ have been tried in lung cancer patients. However, the mechanism of antitumor effect of interferon-γ has not yet been established despite many hypotheses. 'Necrosis' is a type of cell death which is well known to occur in the circumstances of severe stress. In contrast, 'apoptosis' is another type of cell death which occurs in such biological circumstances as embryonic development, regression of organs, and self-tolerance of lymphocytes. And, apoptosis is an active process of cell death in which cells are dying with fragmentations of their cytoplasms and nuclei. And, in the process of apoptosis the DNAs of cells are cleaved between nucleosomes by unidentified endonuclease and therefore DNAs of apoptotic cells result in a typical electrophoresis pattern known as DNA ladder pattern. Recently it has been suggested that cytotoxic effect of interferon-γ occurs via apoptosis. To elucidate the mechanism of antitumor cytotoxic effect of interferon-γ, we microscopically observed a lung cancer cell line, A549 which was treated with interferon-γ. We observed A545 treated with interferon-γ was dying fragmented. And so, we performed this study to find out that the mechanism of antitumor cytotoxic effect of interferon-γ be apoptosis. Method: We treated A549, human lung cancer cell line with various concentrations of interferon-γ and quantified its cytotoxic effect of various periods, 24 hours, 72 hours and, 120 hours by MTT (dimethylthiazolyl diphenyltetrazolium bromide) bioassay. Also, after we treated A549 with 100 units/ml of interferon-γ for 120 hours, we observed the pattern of cell death with inverted microscope and we extracted DNAs from the dead A549 cells and observed the pattern of 1.5% agarose gel electrophoresis with ethidium bromide staining. Result: 1) Cytotoxic effect of interferon-γ on A549: For the first 24 hours, there was little cytotoxic effect and for between 24 hours and 72 hours, there was the beginning of cytotoxic effect and for 120 hours there was increased cytotoxic effect. 2) Pattern of A549 cell death by interferon-γ: We observed with inverted microscope that A549 cells were dying fragmented. 3) DNA ladder pattern of gel electrophoresis: We observed DNA ladder pattern of gel electrophoresis of extracted DNAs from dead A549 cells. Conclusion: We concluded that the mechanism of interferon-γ induced cytotoxicity on lung cancer cell line, A549 be via apoptosis.

AB - Background: Interferon-γ has various biologic effects, including antiviral effect, antitumor proliferative effect, activation of macrophage and B lymphocyte, and increased expression of major histocompatibility complex. Especially, antitumor proliferative effect of interferon-γ has already been proved to be important in vivo as well as in vitro. And, clinical studies of interferon-γ have been tried in lung cancer patients. However, the mechanism of antitumor effect of interferon-γ has not yet been established despite many hypotheses. 'Necrosis' is a type of cell death which is well known to occur in the circumstances of severe stress. In contrast, 'apoptosis' is another type of cell death which occurs in such biological circumstances as embryonic development, regression of organs, and self-tolerance of lymphocytes. And, apoptosis is an active process of cell death in which cells are dying with fragmentations of their cytoplasms and nuclei. And, in the process of apoptosis the DNAs of cells are cleaved between nucleosomes by unidentified endonuclease and therefore DNAs of apoptotic cells result in a typical electrophoresis pattern known as DNA ladder pattern. Recently it has been suggested that cytotoxic effect of interferon-γ occurs via apoptosis. To elucidate the mechanism of antitumor cytotoxic effect of interferon-γ, we microscopically observed a lung cancer cell line, A549 which was treated with interferon-γ. We observed A545 treated with interferon-γ was dying fragmented. And so, we performed this study to find out that the mechanism of antitumor cytotoxic effect of interferon-γ be apoptosis. Method: We treated A549, human lung cancer cell line with various concentrations of interferon-γ and quantified its cytotoxic effect of various periods, 24 hours, 72 hours and, 120 hours by MTT (dimethylthiazolyl diphenyltetrazolium bromide) bioassay. Also, after we treated A549 with 100 units/ml of interferon-γ for 120 hours, we observed the pattern of cell death with inverted microscope and we extracted DNAs from the dead A549 cells and observed the pattern of 1.5% agarose gel electrophoresis with ethidium bromide staining. Result: 1) Cytotoxic effect of interferon-γ on A549: For the first 24 hours, there was little cytotoxic effect and for between 24 hours and 72 hours, there was the beginning of cytotoxic effect and for 120 hours there was increased cytotoxic effect. 2) Pattern of A549 cell death by interferon-γ: We observed with inverted microscope that A549 cells were dying fragmented. 3) DNA ladder pattern of gel electrophoresis: We observed DNA ladder pattern of gel electrophoresis of extracted DNAs from dead A549 cells. Conclusion: We concluded that the mechanism of interferon-γ induced cytotoxicity on lung cancer cell line, A549 be via apoptosis.

KW - A549 and apoptosis

KW - interferon-γ

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