The Eyelid Meibomian Gland Deficiency in Fucosyltransferase 1 Knockout Mice

Chang Ho Yoon, Jin Suk Ryu, Jung Hwa Ko, Yeon Kyung Kim, Jang Hee Oh, Jin Ho Chung, Joo Youn Oh

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate the effect of fucosyltransferase (FUT) 1-mediated fucosylation on meibomian glands (MG), we first confirmed that FUT1 and its fucosylated products were expressed in the eyelid, conjunctiva and skin in wild-type (WT) mice, whereas their mRNA and protein levels were downregulated in Fut1 knock-out (KO) mice. We then evaluated age-dependent changes in the total and acinar areas of MG, meibocyte differentiation, lipid synthesis, and eyelid inflammation and oxidative stress in Fut1 KO and WT mice. Results show that both the total and acinar areas of MG were smaller in Fut1 KO mice than in WT mice in all evaluated age groups. Meibocyte differentiation, lipid-producing capacities and the enzyme levels responsible for lipid synthesis were reduced in Fut1 KO mice, compared to WT controls. The levels of pro-inflammatory cytokines and oxidative-stress-related markers were elevated in the eyelids and MG of FUT1 KO mice. These findings demonstrate the physiologic function of FUT1-mediated fucosylation in MG development and function, and indicate its potential role in ocular surface homeostasis.

Original languageEnglish
Article number9464
JournalInternational Journal of Molecular Sciences
Volume23
Issue number16
DOIs
StatePublished - Aug 2022

Keywords

  • eyelid
  • fucosylation
  • fucosyltransferase 1
  • glycosylation
  • inflammation
  • meibomian gland
  • ocular surface
  • oxidative stress

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