Tat-APE1/ref-1 protein inhibits TNF-α-induced endothelial cell activation

Yun Jeong Song, Ji Young Lee, Hee Kyoung Joo, Hyo Shin Kim, Sang Ki Lee, Kwon Ho Lee, Chung-Hyun Cho, Jin Bong Park, Byeong Hwa Jeon

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/ref-1) is a multifunctional protein involved both in DNA base excision repair and redox regulation. In this study we evaluated the protective role of Tat-mediated APE1/ref-1 transduction on the tumor necrosis factor (TNF)-α-activated endothelial activation in cultured human umbilical vein endothelial cells. To construct Tat-APE1/ref-1 fusion protein, human full length of APE1/ref-1 was fused with Tat-protein transduction domain. Purified Tat-APE1/ref-1 fusion protein efficiently transduced cultured endothelial cells in a dose-dependent manner and reached maximum expression at 1 h after incubation. Transduced Tat-APE1/ref-1 showed inhibitory activity on the TNF-α-induced monocyte adhesion and vascular cell adhesion molecule-1 expression in cultured endothelial cells. These results suggest Tat-APE1/ref-1 might be useful to reduce vascular endothelial activation or vascular inflammatory disorders.

Original languageEnglish
Pages (from-to)68-73
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume368
Issue number1
DOIs
StatePublished - 28 Mar 2008

Keywords

  • Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/ref-1)
  • Endothelial cells
  • Monocyte adhesion
  • Tat-mediated transduction
  • Vascular cell adhesion molecule-1
  • Vascular endothelial activation

Cite this

Song, Y. J., Lee, J. Y., Joo, H. K., Kim, H. S., Lee, S. K., Lee, K. H., Cho, C-H., Park, J. B., & Jeon, B. H. (2008). Tat-APE1/ref-1 protein inhibits TNF-α-induced endothelial cell activation. Biochemical and Biophysical Research Communications, 368(1), 68-73. https://doi.org/10.1016/j.bbrc.2008.01.037