Synergistic antitumor effect of triptolide and cisplatin in cisplatin resistant human bladder cancer cells

Jin Nyoung Ho, Seok-Soo Byun, Sang Chul Lee, Jong Jin Oh, Sung Kyu Hong, Sang Eun Lee, Jae Seung Yeon

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Purpose: We examined the synergistic antitumor effects of cisplatin combined with triptolide in cisplatin resistant T24R2 bladder cancer cells and investigated possible molecular mechanisms. Materials and Methods: T24R2 cells were treated with cisplatin and/or triptolide. Tumor cell proliferation was determined using cell counting and clonogenic assays. The combination index of the synergism between cisplatin and triptolide was calculated. The cell cycle phase and apoptosis rate were determined by flow cytometry. Apoptosis and cell cycle related protein expression were analyzed by Western blot. Results: The synergistic cytotoxicity effect of cisplatin and triptolide combination treatment was greater than the cytotoxic effect of cisplatin or triptolide alone. Combination treatment also induced cell cycle arrest via cyclin D1 and E1 expression. Apoptosis induced by combination treatment was accompanied by increased expression of caspase-3, 8 and 9, PARP and cytochrome c. Conclusions: Our results suggest that triptolide synergistically enhanced the antitumor effect of cisplatin in cisplatin resistant human bladder cancer cells. Cisplatin and triptolide combination treatment may be effective for advanced bladder cancer.

Original languageEnglish
Pages (from-to)1016-1022
Number of pages7
JournalJournal of Urology
Volume193
Issue number3
DOIs
StatePublished - 1 Mar 2015

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Urinary Bladder Neoplasms
Cisplatin
Apoptosis
Colony-Forming Units Assay
triptolide
Cell Cycle Proteins
Caspase 9
Caspase 8
Cyclin D1
Cell Cycle Checkpoints
Cytochromes c
Caspase 3
Cell Cycle
Flow Cytometry
Western Blotting
Cell Proliferation

Keywords

  • cisplatin
  • drug resistance
  • drug therapy, combination
  • triptolide
  • urinary bladder neoplasms

Cite this

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title = "Synergistic antitumor effect of triptolide and cisplatin in cisplatin resistant human bladder cancer cells",
abstract = "Purpose: We examined the synergistic antitumor effects of cisplatin combined with triptolide in cisplatin resistant T24R2 bladder cancer cells and investigated possible molecular mechanisms. Materials and Methods: T24R2 cells were treated with cisplatin and/or triptolide. Tumor cell proliferation was determined using cell counting and clonogenic assays. The combination index of the synergism between cisplatin and triptolide was calculated. The cell cycle phase and apoptosis rate were determined by flow cytometry. Apoptosis and cell cycle related protein expression were analyzed by Western blot. Results: The synergistic cytotoxicity effect of cisplatin and triptolide combination treatment was greater than the cytotoxic effect of cisplatin or triptolide alone. Combination treatment also induced cell cycle arrest via cyclin D1 and E1 expression. Apoptosis induced by combination treatment was accompanied by increased expression of caspase-3, 8 and 9, PARP and cytochrome c. Conclusions: Our results suggest that triptolide synergistically enhanced the antitumor effect of cisplatin in cisplatin resistant human bladder cancer cells. Cisplatin and triptolide combination treatment may be effective for advanced bladder cancer.",
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Synergistic antitumor effect of triptolide and cisplatin in cisplatin resistant human bladder cancer cells. / Ho, Jin Nyoung; Byun, Seok-Soo; Lee, Sang Chul; Oh, Jong Jin; Hong, Sung Kyu; Lee, Sang Eun; Yeon, Jae Seung.

In: Journal of Urology, Vol. 193, No. 3, 01.03.2015, p. 1016-1022.

Research output: Contribution to journalArticle

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AU - Hong, Sung Kyu

AU - Lee, Sang Eun

AU - Yeon, Jae Seung

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N2 - Purpose: We examined the synergistic antitumor effects of cisplatin combined with triptolide in cisplatin resistant T24R2 bladder cancer cells and investigated possible molecular mechanisms. Materials and Methods: T24R2 cells were treated with cisplatin and/or triptolide. Tumor cell proliferation was determined using cell counting and clonogenic assays. The combination index of the synergism between cisplatin and triptolide was calculated. The cell cycle phase and apoptosis rate were determined by flow cytometry. Apoptosis and cell cycle related protein expression were analyzed by Western blot. Results: The synergistic cytotoxicity effect of cisplatin and triptolide combination treatment was greater than the cytotoxic effect of cisplatin or triptolide alone. Combination treatment also induced cell cycle arrest via cyclin D1 and E1 expression. Apoptosis induced by combination treatment was accompanied by increased expression of caspase-3, 8 and 9, PARP and cytochrome c. Conclusions: Our results suggest that triptolide synergistically enhanced the antitumor effect of cisplatin in cisplatin resistant human bladder cancer cells. Cisplatin and triptolide combination treatment may be effective for advanced bladder cancer.

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