Syndecan-2 functions as a docking receptor for pro-matrix metalloproteinase-7 in human colon cancer cells

Heui Young Ryu, Jiseon Lee, Sanghwa Yang, Haein Park, Sojoong Choi, Kyeong Cheon Jung, Seung Taek Lee, Je Kyung Seong, Inn Oc Han, Eok Soo Oh

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Abstract

Although elevated syndecan-2 expression is known to be crucial for the tumorigenic activity in colon carcinoma cells, how syndecan-2 regulates colon cancer is unclear. In human colon adenocarcinoma tissue samples, we found that both mRNA and protein expression of syndecan-2 were increased, compared with the neighboring normal epithelium, suggesting that syndecan-2 plays functional roles in human colon cancer cells. Consistent with this notion, syndecan-2-overexpressing HT-29 colon adenocarcinoma cells showed enhanced migration/invasion, anchorage-independent growth, and primary tumor formation in nude mice, paralleling their morphological changes into highly tumorigenic cells. In addition, our experiments revealed that syndecan-2 enhanced both expression and secretion of matrix metalloproteinase-7 (MMP-7), directly interacted with pro-MMP-7, and potentiated the enzymatic activity of pro-MMP-7 by activating its processing into the active MMP-7. Collectively, these data strongly suggest that syndecan-2 functions as a docking receptor for pro-MMP-7 in colon cancer cells.

Original languageEnglish
Pages (from-to)35692-35701
Number of pages10
JournalJournal of Biological Chemistry
Volume284
Issue number51
DOIs
StatePublished - 18 Dec 2009

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Syndecan-2
Matrix Metalloproteinase 7
Colonic Neoplasms
Cells
Colon
Adenocarcinoma
Nude Mice
Tumors
Epithelium
Tissue
Carcinoma
Messenger RNA

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Ryu, Heui Young ; Lee, Jiseon ; Yang, Sanghwa ; Park, Haein ; Choi, Sojoong ; Jung, Kyeong Cheon ; Lee, Seung Taek ; Seong, Je Kyung ; Han, Inn Oc ; Oh, Eok Soo. / Syndecan-2 functions as a docking receptor for pro-matrix metalloproteinase-7 in human colon cancer cells. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 51. pp. 35692-35701.
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abstract = "Although elevated syndecan-2 expression is known to be crucial for the tumorigenic activity in colon carcinoma cells, how syndecan-2 regulates colon cancer is unclear. In human colon adenocarcinoma tissue samples, we found that both mRNA and protein expression of syndecan-2 were increased, compared with the neighboring normal epithelium, suggesting that syndecan-2 plays functional roles in human colon cancer cells. Consistent with this notion, syndecan-2-overexpressing HT-29 colon adenocarcinoma cells showed enhanced migration/invasion, anchorage-independent growth, and primary tumor formation in nude mice, paralleling their morphological changes into highly tumorigenic cells. In addition, our experiments revealed that syndecan-2 enhanced both expression and secretion of matrix metalloproteinase-7 (MMP-7), directly interacted with pro-MMP-7, and potentiated the enzymatic activity of pro-MMP-7 by activating its processing into the active MMP-7. Collectively, these data strongly suggest that syndecan-2 functions as a docking receptor for pro-MMP-7 in colon cancer cells.",
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Ryu, HY, Lee, J, Yang, S, Park, H, Choi, S, Jung, KC, Lee, ST, Seong, JK, Han, IO & Oh, ES 2009, 'Syndecan-2 functions as a docking receptor for pro-matrix metalloproteinase-7 in human colon cancer cells', Journal of Biological Chemistry, vol. 284, no. 51, pp. 35692-35701. https://doi.org/10.1074/jbc.M109.054254

Syndecan-2 functions as a docking receptor for pro-matrix metalloproteinase-7 in human colon cancer cells. / Ryu, Heui Young; Lee, Jiseon; Yang, Sanghwa; Park, Haein; Choi, Sojoong; Jung, Kyeong Cheon; Lee, Seung Taek; Seong, Je Kyung; Han, Inn Oc; Oh, Eok Soo.

In: Journal of Biological Chemistry, Vol. 284, No. 51, 18.12.2009, p. 35692-35701.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Ryu, Heui Young

AU - Lee, Jiseon

AU - Yang, Sanghwa

AU - Park, Haein

AU - Choi, Sojoong

AU - Jung, Kyeong Cheon

AU - Lee, Seung Taek

AU - Seong, Je Kyung

AU - Han, Inn Oc

AU - Oh, Eok Soo

PY - 2009/12/18

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AB - Although elevated syndecan-2 expression is known to be crucial for the tumorigenic activity in colon carcinoma cells, how syndecan-2 regulates colon cancer is unclear. In human colon adenocarcinoma tissue samples, we found that both mRNA and protein expression of syndecan-2 were increased, compared with the neighboring normal epithelium, suggesting that syndecan-2 plays functional roles in human colon cancer cells. Consistent with this notion, syndecan-2-overexpressing HT-29 colon adenocarcinoma cells showed enhanced migration/invasion, anchorage-independent growth, and primary tumor formation in nude mice, paralleling their morphological changes into highly tumorigenic cells. In addition, our experiments revealed that syndecan-2 enhanced both expression and secretion of matrix metalloproteinase-7 (MMP-7), directly interacted with pro-MMP-7, and potentiated the enzymatic activity of pro-MMP-7 by activating its processing into the active MMP-7. Collectively, these data strongly suggest that syndecan-2 functions as a docking receptor for pro-MMP-7 in colon cancer cells.

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