Suppression of the carbachol-activated nonselective cationic current by antibody against alpha subunit of G(o) protein in guinea-pig gastric myocytes

Young Chul Kim, Sungjoon Kim, Jae Hoon Sim, Chin Ho Cho, Yong-Sung Juhnn, Suk Hyo Suh, Insuk So, Ki Whan Kim

Research output: Contribution to journalArticleResearchpeer-review

42 Citations (Scopus)

Abstract

In this study, we investigated which subtype of GTP-binding protein (G protein) is related to muscarinic activation of nonselective cation (NSC) channels in gastric smooth muscle. Inward cationic current was activated by the application of 50 pM carbachol (I(CCh)) at a holding potential of -60 mV with the same CsCl-rich solution in both pipette and bath. The same cationic current as I(CCh) was slowly activated by the dialysis of guanosine 5'-O-(3- thiotriphosphate) (GTP[γ-S]) through the pipette. Since it is known that pertussis toxin pretreatment can block I(CCh), antibodies (Abs) against G(α,i) (anti-G(α,i)) or G(α,o) (anti-G(α,o)) were tested. Activation of I(CCh) was blocked by the addition of anti-G(α,o). However, anti-G(α,i) Abs had no significant effect on I(CCh). The expression of G(α,o) in guinea-pig gastric smooth muscle was confirmed by Western immunoblot analysis. These results suggest that G(o)-type protein may mediate signals from the muscarinic receptor to NSC channel in guinea-pig gastric myocytes.

Original languageEnglish
Pages (from-to)494-496
Number of pages3
JournalPflugers Archiv European Journal of Physiology
Volume436
Issue number3
DOIs
StatePublished - 27 Jul 1998

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Gi-Go GTP-Binding Protein alpha Subunits
Carbachol
Muscle Cells
Stomach
Guinea Pigs
Antibodies
GTP-Binding Proteins
Smooth Muscle
Muscle
Cations
Chemical activation
Guanosine 5'-O-(3-Thiotriphosphate)
Dialysis
Pertussis Toxin
Muscarinic Receptors
Guanosine Triphosphate
Baths
Cholinergic Agents
Western Blotting

Keywords

  • Carbachol (CCh)
  • G protein
  • Nonselective cationic current
  • Smooth muscle

Cite this

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title = "Suppression of the carbachol-activated nonselective cationic current by antibody against alpha subunit of G(o) protein in guinea-pig gastric myocytes",
abstract = "In this study, we investigated which subtype of GTP-binding protein (G protein) is related to muscarinic activation of nonselective cation (NSC) channels in gastric smooth muscle. Inward cationic current was activated by the application of 50 pM carbachol (I(CCh)) at a holding potential of -60 mV with the same CsCl-rich solution in both pipette and bath. The same cationic current as I(CCh) was slowly activated by the dialysis of guanosine 5'-O-(3- thiotriphosphate) (GTP[γ-S]) through the pipette. Since it is known that pertussis toxin pretreatment can block I(CCh), antibodies (Abs) against G(α,i) (anti-G(α,i)) or G(α,o) (anti-G(α,o)) were tested. Activation of I(CCh) was blocked by the addition of anti-G(α,o). However, anti-G(α,i) Abs had no significant effect on I(CCh). The expression of G(α,o) in guinea-pig gastric smooth muscle was confirmed by Western immunoblot analysis. These results suggest that G(o)-type protein may mediate signals from the muscarinic receptor to NSC channel in guinea-pig gastric myocytes.",
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Suppression of the carbachol-activated nonselective cationic current by antibody against alpha subunit of G(o) protein in guinea-pig gastric myocytes. / Kim, Young Chul; Kim, Sungjoon; Sim, Jae Hoon; Cho, Chin Ho; Juhnn, Yong-Sung; Suh, Suk Hyo; So, Insuk; Kim, Ki Whan.

In: Pflugers Archiv European Journal of Physiology, Vol. 436, No. 3, 27.07.1998, p. 494-496.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Suppression of the carbachol-activated nonselective cationic current by antibody against alpha subunit of G(o) protein in guinea-pig gastric myocytes

AU - Kim, Young Chul

AU - Kim, Sungjoon

AU - Sim, Jae Hoon

AU - Cho, Chin Ho

AU - Juhnn, Yong-Sung

AU - Suh, Suk Hyo

AU - So, Insuk

AU - Kim, Ki Whan

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Y1 - 1998/7/27

N2 - In this study, we investigated which subtype of GTP-binding protein (G protein) is related to muscarinic activation of nonselective cation (NSC) channels in gastric smooth muscle. Inward cationic current was activated by the application of 50 pM carbachol (I(CCh)) at a holding potential of -60 mV with the same CsCl-rich solution in both pipette and bath. The same cationic current as I(CCh) was slowly activated by the dialysis of guanosine 5'-O-(3- thiotriphosphate) (GTP[γ-S]) through the pipette. Since it is known that pertussis toxin pretreatment can block I(CCh), antibodies (Abs) against G(α,i) (anti-G(α,i)) or G(α,o) (anti-G(α,o)) were tested. Activation of I(CCh) was blocked by the addition of anti-G(α,o). However, anti-G(α,i) Abs had no significant effect on I(CCh). The expression of G(α,o) in guinea-pig gastric smooth muscle was confirmed by Western immunoblot analysis. These results suggest that G(o)-type protein may mediate signals from the muscarinic receptor to NSC channel in guinea-pig gastric myocytes.

AB - In this study, we investigated which subtype of GTP-binding protein (G protein) is related to muscarinic activation of nonselective cation (NSC) channels in gastric smooth muscle. Inward cationic current was activated by the application of 50 pM carbachol (I(CCh)) at a holding potential of -60 mV with the same CsCl-rich solution in both pipette and bath. The same cationic current as I(CCh) was slowly activated by the dialysis of guanosine 5'-O-(3- thiotriphosphate) (GTP[γ-S]) through the pipette. Since it is known that pertussis toxin pretreatment can block I(CCh), antibodies (Abs) against G(α,i) (anti-G(α,i)) or G(α,o) (anti-G(α,o)) were tested. Activation of I(CCh) was blocked by the addition of anti-G(α,o). However, anti-G(α,i) Abs had no significant effect on I(CCh). The expression of G(α,o) in guinea-pig gastric smooth muscle was confirmed by Western immunoblot analysis. These results suggest that G(o)-type protein may mediate signals from the muscarinic receptor to NSC channel in guinea-pig gastric myocytes.

KW - Carbachol (CCh)

KW - G protein

KW - Nonselective cationic current

KW - Smooth muscle

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