Study on IL-8 expression in peripheral blood monocytes

J. Y. Kim, J. C. Lee, M. J. Kang, Jong Sun Park, Chul Gyu Yoo, Youngwhan Kim, Sungkoo Han, Y. S. Shim

Research output: Contribution to journalArticle

Abstract

Background: Peripheral blood monocytes are important immune effector cells that play a fundamental role in cellular immunity. In addition to their antigen-presenting and phagocytic activities, monocytes/macrophage produce a vast array of regulatory and chemotactic cytokines. Interleukin-8 (IL-8), a potent neutrophil-activating and chemotactic peptide, is produced in large quantities by mononuclear phagocytes and may be an important mediator of local and systemic inflammation. Overexpression by IL-8 of such inflammation may be an important step of tissue injury frequently seen in inflammatory reaction. So it could be hypothesized that the agents which block the production of IL-8 can decrease the inflammatory reaction and tissue injury. To evaluate this, we described the effect of Dexamethasone, PGE2, Indomethacin and Interferon-γ (IFN-γ) on IL-8 mRNA and protein expression from LPS-stimulated human peripheral blood monocytes (PBMC). Method: PBMC was isolated from healthy volunteers. To evaluate the effect of Dexamethasone, PGE2 and Indomethacin, these drug were treated for 1 hour before and after LPS stimulation and IFN-γ was only treated 1 hour before the LPS stimulation. Northern blot analysis for IL-8 mRNA and ELISA for immunoreactive IL-8 protein in culture supernatant were performed. We repeated above experiment three times for Northern blot analysis and two times for ELISA and got the same result. Results: 1) Pre- and post-treatment of Dexamethasone suppressed both the LPS stimulated IL-8 mRNA expression and IL-8 protein release in PBMC. 2) IFN-γ pre-treatment suppressed the IL-8 mRNA expression and IL-8 protein release in unstimulated cells. 3) In LPS stimulated cells, IFN-γ suppressed the IL-8 mRNA expression but IL-8 protein release suppression was not observed. 4) PGE2 and Indomethacin exert no effect on the LPS-stimulated IL-8 mRNA and protein expression in concentration used in this experiment (PGE2; 10-6 M, Indomethacin; 10 μM). Conclusion: One of the mechanisms of antiinflammatory action of Dexamethasone can be explained by the suppressing effect of IL-8 production in some extent and by this antiinflammatory effect, dexamethasone can be used to suppress local and systemic inflammation mediated by IL-8.

Original languageEnglish
Pages (from-to)703-712
Number of pages10
JournalTuberculosis and Respiratory Diseases
Volume42
Issue number5
DOIs
StatePublished - 1 Jan 1995

Fingerprint

Interleukin-8
Monocytes
Dexamethasone
Dinoprostone
Indomethacin
Messenger RNA
Interferons
Proteins
Inflammation
Northern Blotting
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay
Wounds and Injuries
Phagocytes
Chemokines
Cellular Immunity
Healthy Volunteers
Neutrophils
Therapeutics
Macrophages

Keywords

  • dexamethasone
  • IL-8
  • indomethacin
  • interferon-γ
  • LPS
  • PBMC
  • PGE

Cite this

@article{c0e602e31524490fba6eda24cde7879e,
title = "Study on IL-8 expression in peripheral blood monocytes",
abstract = "Background: Peripheral blood monocytes are important immune effector cells that play a fundamental role in cellular immunity. In addition to their antigen-presenting and phagocytic activities, monocytes/macrophage produce a vast array of regulatory and chemotactic cytokines. Interleukin-8 (IL-8), a potent neutrophil-activating and chemotactic peptide, is produced in large quantities by mononuclear phagocytes and may be an important mediator of local and systemic inflammation. Overexpression by IL-8 of such inflammation may be an important step of tissue injury frequently seen in inflammatory reaction. So it could be hypothesized that the agents which block the production of IL-8 can decrease the inflammatory reaction and tissue injury. To evaluate this, we described the effect of Dexamethasone, PGE2, Indomethacin and Interferon-γ (IFN-γ) on IL-8 mRNA and protein expression from LPS-stimulated human peripheral blood monocytes (PBMC). Method: PBMC was isolated from healthy volunteers. To evaluate the effect of Dexamethasone, PGE2 and Indomethacin, these drug were treated for 1 hour before and after LPS stimulation and IFN-γ was only treated 1 hour before the LPS stimulation. Northern blot analysis for IL-8 mRNA and ELISA for immunoreactive IL-8 protein in culture supernatant were performed. We repeated above experiment three times for Northern blot analysis and two times for ELISA and got the same result. Results: 1) Pre- and post-treatment of Dexamethasone suppressed both the LPS stimulated IL-8 mRNA expression and IL-8 protein release in PBMC. 2) IFN-γ pre-treatment suppressed the IL-8 mRNA expression and IL-8 protein release in unstimulated cells. 3) In LPS stimulated cells, IFN-γ suppressed the IL-8 mRNA expression but IL-8 protein release suppression was not observed. 4) PGE2 and Indomethacin exert no effect on the LPS-stimulated IL-8 mRNA and protein expression in concentration used in this experiment (PGE2; 10-6 M, Indomethacin; 10 μM). Conclusion: One of the mechanisms of antiinflammatory action of Dexamethasone can be explained by the suppressing effect of IL-8 production in some extent and by this antiinflammatory effect, dexamethasone can be used to suppress local and systemic inflammation mediated by IL-8.",
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author = "Kim, {J. Y.} and Lee, {J. C.} and Kang, {M. J.} and Park, {Jong Sun} and Yoo, {Chul Gyu} and Youngwhan Kim and Sungkoo Han and Shim, {Y. S.}",
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Study on IL-8 expression in peripheral blood monocytes. / Kim, J. Y.; Lee, J. C.; Kang, M. J.; Park, Jong Sun; Yoo, Chul Gyu; Kim, Youngwhan; Han, Sungkoo; Shim, Y. S.

In: Tuberculosis and Respiratory Diseases, Vol. 42, No. 5, 01.01.1995, p. 703-712.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Study on IL-8 expression in peripheral blood monocytes

AU - Kim, J. Y.

AU - Lee, J. C.

AU - Kang, M. J.

AU - Park, Jong Sun

AU - Yoo, Chul Gyu

AU - Kim, Youngwhan

AU - Han, Sungkoo

AU - Shim, Y. S.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Background: Peripheral blood monocytes are important immune effector cells that play a fundamental role in cellular immunity. In addition to their antigen-presenting and phagocytic activities, monocytes/macrophage produce a vast array of regulatory and chemotactic cytokines. Interleukin-8 (IL-8), a potent neutrophil-activating and chemotactic peptide, is produced in large quantities by mononuclear phagocytes and may be an important mediator of local and systemic inflammation. Overexpression by IL-8 of such inflammation may be an important step of tissue injury frequently seen in inflammatory reaction. So it could be hypothesized that the agents which block the production of IL-8 can decrease the inflammatory reaction and tissue injury. To evaluate this, we described the effect of Dexamethasone, PGE2, Indomethacin and Interferon-γ (IFN-γ) on IL-8 mRNA and protein expression from LPS-stimulated human peripheral blood monocytes (PBMC). Method: PBMC was isolated from healthy volunteers. To evaluate the effect of Dexamethasone, PGE2 and Indomethacin, these drug were treated for 1 hour before and after LPS stimulation and IFN-γ was only treated 1 hour before the LPS stimulation. Northern blot analysis for IL-8 mRNA and ELISA for immunoreactive IL-8 protein in culture supernatant were performed. We repeated above experiment three times for Northern blot analysis and two times for ELISA and got the same result. Results: 1) Pre- and post-treatment of Dexamethasone suppressed both the LPS stimulated IL-8 mRNA expression and IL-8 protein release in PBMC. 2) IFN-γ pre-treatment suppressed the IL-8 mRNA expression and IL-8 protein release in unstimulated cells. 3) In LPS stimulated cells, IFN-γ suppressed the IL-8 mRNA expression but IL-8 protein release suppression was not observed. 4) PGE2 and Indomethacin exert no effect on the LPS-stimulated IL-8 mRNA and protein expression in concentration used in this experiment (PGE2; 10-6 M, Indomethacin; 10 μM). Conclusion: One of the mechanisms of antiinflammatory action of Dexamethasone can be explained by the suppressing effect of IL-8 production in some extent and by this antiinflammatory effect, dexamethasone can be used to suppress local and systemic inflammation mediated by IL-8.

AB - Background: Peripheral blood monocytes are important immune effector cells that play a fundamental role in cellular immunity. In addition to their antigen-presenting and phagocytic activities, monocytes/macrophage produce a vast array of regulatory and chemotactic cytokines. Interleukin-8 (IL-8), a potent neutrophil-activating and chemotactic peptide, is produced in large quantities by mononuclear phagocytes and may be an important mediator of local and systemic inflammation. Overexpression by IL-8 of such inflammation may be an important step of tissue injury frequently seen in inflammatory reaction. So it could be hypothesized that the agents which block the production of IL-8 can decrease the inflammatory reaction and tissue injury. To evaluate this, we described the effect of Dexamethasone, PGE2, Indomethacin and Interferon-γ (IFN-γ) on IL-8 mRNA and protein expression from LPS-stimulated human peripheral blood monocytes (PBMC). Method: PBMC was isolated from healthy volunteers. To evaluate the effect of Dexamethasone, PGE2 and Indomethacin, these drug were treated for 1 hour before and after LPS stimulation and IFN-γ was only treated 1 hour before the LPS stimulation. Northern blot analysis for IL-8 mRNA and ELISA for immunoreactive IL-8 protein in culture supernatant were performed. We repeated above experiment three times for Northern blot analysis and two times for ELISA and got the same result. Results: 1) Pre- and post-treatment of Dexamethasone suppressed both the LPS stimulated IL-8 mRNA expression and IL-8 protein release in PBMC. 2) IFN-γ pre-treatment suppressed the IL-8 mRNA expression and IL-8 protein release in unstimulated cells. 3) In LPS stimulated cells, IFN-γ suppressed the IL-8 mRNA expression but IL-8 protein release suppression was not observed. 4) PGE2 and Indomethacin exert no effect on the LPS-stimulated IL-8 mRNA and protein expression in concentration used in this experiment (PGE2; 10-6 M, Indomethacin; 10 μM). Conclusion: One of the mechanisms of antiinflammatory action of Dexamethasone can be explained by the suppressing effect of IL-8 production in some extent and by this antiinflammatory effect, dexamethasone can be used to suppress local and systemic inflammation mediated by IL-8.

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KW - IL-8

KW - indomethacin

KW - interferon-γ

KW - LPS

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KW - PGE

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