Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: Insight into the basis of its substrate specificity

Youngsoo Kim, Wim G.J. Hol

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Background: Semisynthetic cephalosporins are primarily synthesized from 7-aminocephalosporanic acid (7-ACA), which is obtained by environmentally toxic chemical deacylation of cephalosporin C (CPC). Thus, the enzymatic conversion of CPC to 7-ACA by cephalosporin acylase (CA) would be of great interest. However, CAs use glutaryl-7-ACA (GL-7-ACA) as a primary substrate and the enzyme has low turnover rates for CPC. Results: The binary complex structures of CA with GL-7-ACA and glutarate (the side-chain of GL-7-ACA) show extensive interactions between the glutaryl moiety of GL-7-ACA and the seven residues that form the side-chain pocket. These interactions explain why the D-α-aminoadipyl side-chain of CPC yields a poorer substrate than GL-7-ACA. Conclusions: This understanding of the nature of substrate specificity may be useful in the design of an enzyme with an improved performance for the conversion of CPC to 7-ACA. Additionally, the catalytic mechanism of the deacylation reaction was revealed by the ligand bound structures.

Original languageEnglish
Pages (from-to)1253-1264
Number of pages12
JournalChemistry and Biology
Volume8
Issue number12
DOIs
StatePublished - 2001

Keywords

  • Cephalosporin acylase
  • Cephalosporin antibiotic
  • Glutaryl-7-aminocephalosporanic acid
  • Substrate specificity

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