Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell-selectin interaction kinetics

Hao Cheng, Marta Byrska-Bishop, Cathy T. Zhang, Christian J. Kastrup, Nathaniel S. Hwang, Albert K. Tai, Won Woo Lee, Xiaoyang Xu, Matthias Nahrendorf, Robert Langer, Daniel G. Anderson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Dynamic cell-microenvironment interactions regulate many biological events and play a critical role in tissue regeneration. Cell homing to targeted tissues requires well balanced interactions between cells and adhesion molecules on blood vessel walls. However, many stem cells lack affinity with adhesion molecules. It is challenging and clinically important to engineer these stem cells to modulate their dynamic interactions with blood vessels. In this study, a new chemical strategy was developed to engineer cell-microenvironment interactions. This method allowed the conjugation of peptides onto stem cell membranes without affecting cell viability, proliferation or multipotency. Mesenchymal stem cells (MSCs) engineered in this manner showed controlled firm adhesion and rolling on E-selectin under physiological shear stresses. For the first time, these biomechanical responses were achieved by tuning the binding kinetics of the peptide-selectin interaction. Rolling of engineered MSCs on E-selectin is mediated by a Ca 2+ independent interaction, a mechanism that differs from the Ca 2+ dependent physiological process. This further illustrates the ability of this approach to manipulate cell-microenvironment interactions, in particular for the application of delivering cells to targeted tissues. It also provides a new platform to engineer cells with multiple functionalities.

Original languageEnglish
Pages (from-to)5004-5012
Number of pages9
JournalBiomaterials
Volume33
Issue number20
DOIs
StatePublished - 1 Jul 2012

Fingerprint

Cell Engineering
Cellular Microenvironment
Selectins
Cell membranes
Stem cells
Cell Communication
Peptides
E-Selectin
Stem Cells
Tuning
Cell Membrane
Mesenchymal Stromal Cells
Kinetics
Blood Vessels
Adhesion
Physiological Phenomena
Blood vessels
Engineers
Cell Adhesion Molecules
Regeneration

Keywords

  • Bioorthogonal chemistry
  • Cell engineering
  • Cell surface
  • Inflammation
  • Myocardial infarction

Cite this

Cheng, H., Byrska-Bishop, M., Zhang, C. T., Kastrup, C. J., Hwang, N. S., Tai, A. K., ... Anderson, D. G. (2012). Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell-selectin interaction kinetics. Biomaterials, 33(20), 5004-5012. https://doi.org/10.1016/j.biomaterials.2012.03.065
Cheng, Hao ; Byrska-Bishop, Marta ; Zhang, Cathy T. ; Kastrup, Christian J. ; Hwang, Nathaniel S. ; Tai, Albert K. ; Lee, Won Woo ; Xu, Xiaoyang ; Nahrendorf, Matthias ; Langer, Robert ; Anderson, Daniel G. / Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell-selectin interaction kinetics. In: Biomaterials. 2012 ; Vol. 33, No. 20. pp. 5004-5012.
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Cheng, H, Byrska-Bishop, M, Zhang, CT, Kastrup, CJ, Hwang, NS, Tai, AK, Lee, WW, Xu, X, Nahrendorf, M, Langer, R & Anderson, DG 2012, 'Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell-selectin interaction kinetics', Biomaterials, vol. 33, no. 20, pp. 5004-5012. https://doi.org/10.1016/j.biomaterials.2012.03.065

Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell-selectin interaction kinetics. / Cheng, Hao; Byrska-Bishop, Marta; Zhang, Cathy T.; Kastrup, Christian J.; Hwang, Nathaniel S.; Tai, Albert K.; Lee, Won Woo; Xu, Xiaoyang; Nahrendorf, Matthias; Langer, Robert; Anderson, Daniel G.

In: Biomaterials, Vol. 33, No. 20, 01.07.2012, p. 5004-5012.

Research output: Contribution to journalArticle

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AU - Zhang, Cathy T.

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AU - Hwang, Nathaniel S.

AU - Tai, Albert K.

AU - Lee, Won Woo

AU - Xu, Xiaoyang

AU - Nahrendorf, Matthias

AU - Langer, Robert

AU - Anderson, Daniel G.

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