Sperm DNA fragmentation in consecutive ejaculates from patients with cancer for sperm cryopreservation

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Objective: This prospective consecutive study investigated the variation in sperm DNA fragmentation (SDF) in multiple semen samples from patients with cancer. Methods: Eighty-one patients with various cancers underwent multiple semen collections on 3 consecutive days for sperm cryopreservation prior to cancer treatment. A commercial Halosperm kit was used to measure SDF. Within and between-subject coefficients of variation were estimated via random-effects analysis of variance to assess the consistency of semen parameters and SDF. Intraclass correlation coefficients (ICCs) were calculated to assess the magnitude of the between-subject component of variance relative to the total variance. Results: The volume of semen in the day-2 and day-3 samples was significantly lower compared with the day-1 sample. Most parameters showed high ICC values, suggesting that within-subject fluctuations were small relative to the between-subject variability. The highest ICC values were identified for the SDF (ICC, 0.68; 95% confidence interval [CI], 0.45–0.84) and semen volume (ICC, 0.67; 95% CI, 0.45–0.84).

Original languageEnglish
Pages (from-to)196-201
Number of pages6
JournalClinical and Experimental Reproductive Medicine
Issue number3
StatePublished - 31 Aug 2022


  • Cryopreservation
  • Dna fragmentation
  • Fertility preservation
  • Semen analysis
  • Sperm


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