Serodiagnostic antigens of clonorchis sinensis identified and evaluated by high-throughput proteogenomics

Pyo Yun Cho, Ji Yun Lee, Tae Im Kim, Jin Ho Song, Sung Jong Hong, Won Gi Yoo, Takafumi Tsuboi, Kwon Soo Ha, Jae Wan Jung, Satoru Takeo, Eun Taek Han, Banchob Sripa, Sung Tae Hong, Jong Yil Chai, Ho Woo Nam, Jhang Ho Pak, Tong Soo Kim

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4 Scopus citations


Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 mil-lion people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C. sinensis-infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini-infected sera. Of the six antigenic proteins selected, four were synthe-sized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.

Original languageEnglish
Article numbere0008998
Pages (from-to)1-15
Number of pages15
JournalPLoS neglected tropical diseases
Issue number12
StatePublished - Dec 2020


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