Selective gene expression in hepatic tumor with trans-arterial delivery of DNA/liposome/transferrin complex

J. G. Seol, D. S. Heo, H. K. Kim, J. H. Yoon, B. I. Choi, H. S. Lee, N. K. Kim, C. Y. Kim

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Since hepatocellular carcinoma (HCC) is frequently presented at an advanced stage, only a small portion of patients with HCC can be treated with local modalities. Gene therapy is, therefore, one of the more promising approaches for patients with advanced HCC. To develop a new strategy for targeting gene delivery to the hepatic tumor, the efficiency of the trans-arterial delivery of liposome-DNA complex was evaluated in VX2 carcinoma implanted into the liver of rabbits. A mixture of pSV-β galactosidase plasmid (40 μg), lipofectin (80 μl), and transferrin (852 μg), the optimal proportion of which determined in vitro, was infused via the hepatic artery of a rabbit with VX2 hepatic tumors. The efficiency of trans-arterial gene delivery was compared to that of intra-tumoral injection. Rabbits (5 in each group) were sacrificed 48 hours after gene delivery and hepatic tissues were examined using X-gal staining. β-galactosidase staining was observed exclusively within the tumor following the trans-arterial gene transfer. In contrast, adjacent peritumoral cells in addition to hepatic tumor cells were transfected by the intra-tumoral injection of transgene. These data indicate that enhanced gene expression in hepatic tumors is possible using trans-arterial delivery of the liposome-DNA complex.

Original languageEnglish
Pages (from-to)513-517
Number of pages5
JournalIn Vivo
Volume14
Issue number4
StatePublished - 23 Aug 2000

Keywords

  • Gene delivery
  • Hepatocellular carcinoma
  • Liposome
  • Trans-arterial
  • VX2 carcinoma

Cite this

Seol, J. G., Heo, D. S., Kim, H. K., Yoon, J. H., Choi, B. I., Lee, H. S., Kim, N. K., & Kim, C. Y. (2000). Selective gene expression in hepatic tumor with trans-arterial delivery of DNA/liposome/transferrin complex. In Vivo, 14(4), 513-517.