Selection of peptides for lipopolysaccharide binding on to epoxy beads and selective detection of Gram-negative bacteria

Yun Gon Kim, Chang Soo Lee, Woo Jae Chung, Eun Mi Kim, Dong Sik Shin, June Hyung Kim, Yoon Sik Lee, Junho Chung, Byung Gee Kim

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Lipopolysaccharide (LPS)-binding peptides were enriched by using epoxy beads as a novel support to immobilize LPS for a phage displayed peptide library screening. The sequence of Phe-Ala-Pro-Trp (FAPW) was the most significant consensus motif of 10 selected clones, and Pro-Phe (PF) was the key dipeptide for binding at the apex of the loop to form a characteristic structure of CXXPFXXXC. Moreover, AWLPWAK, one of the highly conserved heptamer peptides, could detect specifically Gram-negative bacteria via a whole cell binding test at 106 cells ml-1.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalBiotechnology Letters
Volume28
Issue number2
DOIs
StatePublished - 1 Jan 2006

Keywords

  • Bacteria detection
  • Epoxy beads
  • Lipopolysaccharide
  • Peptides
  • Phage display

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