Section directed cryosectioning of specimens for scanning electron microscopy: A new method to study cardiac development

Jeong‐Wook ‐W Seo, Eul‐Kyeong ‐K Kim, Nigel A. Brown, Andy Wessels

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A new method to study the developing heart was developed. Using this “section directed” cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with the scanning electron microscope can be compared in detail with the information on the molecular phenotypes of the subpopulations of cells as deducted from staining patterns of the sections. This method allows combination of the specific advantages of sophisticated histological techniques, such as immunohistochemistry and in situ hybridisation, with those of the scanning electron microscope. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)491-495
Number of pages5
JournalMicroscopy Research and Technique
Issue number6
StatePublished - 15 Apr 1995


  • Heart
  • Immunohistochemistry
  • Scanning electron microscopy
  • “Section directed” cryosectioning

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