Section directed cryosectioning of specimens for scanning electron microscopy

A new method to study cardiac development

Jeong-Wook Seo, Eul‐Kyeong ‐K Kim, Nigel A. Brown, Andy Wessels

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

A new method to study the developing heart was developed. Using this “section directed” cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with the scanning electron microscope can be compared in detail with the information on the molecular phenotypes of the subpopulations of cells as deducted from staining patterns of the sections. This method allows combination of the specific advantages of sophisticated histological techniques, such as immunohistochemistry and in situ hybridisation, with those of the scanning electron microscope. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)491-495
Number of pages5
JournalMicroscopy Research and Technique
Volume30
Issue number6
DOIs
StatePublished - 1 Jan 1995

Fingerprint

Cryoultramicrotomy
Electron Scanning Microscopy
Electron microscopes
Scanning
Scanning electron microscopy
scanning electron microscopy
electron microscopes
Electrons
Histological Techniques
phenotype
scanning
embryos
Cells
staining
In Situ Hybridization
Embryonic Structures
Immunohistochemistry
Staining and Labeling
Phenotype

Keywords

  • Heart
  • Immunohistochemistry
  • Scanning electron microscopy
  • “Section directed” cryosectioning

Cite this

@article{27fb730b969f4507af9f6c912dfa493a,
title = "Section directed cryosectioning of specimens for scanning electron microscopy: A new method to study cardiac development",
abstract = "A new method to study the developing heart was developed. Using this “section directed” cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with the scanning electron microscope can be compared in detail with the information on the molecular phenotypes of the subpopulations of cells as deducted from staining patterns of the sections. This method allows combination of the specific advantages of sophisticated histological techniques, such as immunohistochemistry and in situ hybridisation, with those of the scanning electron microscope. {\circledC} 1995 Wiley‐Liss, Inc.",
keywords = "Heart, Immunohistochemistry, Scanning electron microscopy, “Section directed” cryosectioning",
author = "Jeong-Wook Seo and Kim, {Eul‐Kyeong ‐K} and Brown, {Nigel A.} and Andy Wessels",
year = "1995",
month = "1",
day = "1",
doi = "10.1002/jemt.1070300606",
language = "English",
volume = "30",
pages = "491--495",
journal = "Microscopy Research and Technique",
issn = "1059-910X",
publisher = "Wiley-Liss Inc.",
number = "6",

}

Section directed cryosectioning of specimens for scanning electron microscopy : A new method to study cardiac development. / Seo, Jeong-Wook; Kim, Eul‐Kyeong ‐K; Brown, Nigel A.; Wessels, Andy.

In: Microscopy Research and Technique, Vol. 30, No. 6, 01.01.1995, p. 491-495.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Section directed cryosectioning of specimens for scanning electron microscopy

T2 - A new method to study cardiac development

AU - Seo, Jeong-Wook

AU - Kim, Eul‐Kyeong ‐K

AU - Brown, Nigel A.

AU - Wessels, Andy

PY - 1995/1/1

Y1 - 1995/1/1

N2 - A new method to study the developing heart was developed. Using this “section directed” cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with the scanning electron microscope can be compared in detail with the information on the molecular phenotypes of the subpopulations of cells as deducted from staining patterns of the sections. This method allows combination of the specific advantages of sophisticated histological techniques, such as immunohistochemistry and in situ hybridisation, with those of the scanning electron microscope. © 1995 Wiley‐Liss, Inc.

AB - A new method to study the developing heart was developed. Using this “section directed” cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with the scanning electron microscope can be compared in detail with the information on the molecular phenotypes of the subpopulations of cells as deducted from staining patterns of the sections. This method allows combination of the specific advantages of sophisticated histological techniques, such as immunohistochemistry and in situ hybridisation, with those of the scanning electron microscope. © 1995 Wiley‐Liss, Inc.

KW - Heart

KW - Immunohistochemistry

KW - Scanning electron microscopy

KW - “Section directed” cryosectioning

UR - http://www.scopus.com/inward/record.url?scp=0028926885&partnerID=8YFLogxK

U2 - 10.1002/jemt.1070300606

DO - 10.1002/jemt.1070300606

M3 - Article

VL - 30

SP - 491

EP - 495

JO - Microscopy Research and Technique

JF - Microscopy Research and Technique

SN - 1059-910X

IS - 6

ER -