Screening of LPS-specific peptides from a phage display library using epoxy beads

Yun Gon Kim, Chang Soo Lee, Woo Jae Chung, Eun Mi Kim, Dong Sik Shin, Jung Hyo Rhim, Yoon Sik Lee, Byung Gee Kim, Junho Chung

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The selection of identical or highly homologous peptides from phage display combinatorial peptide libraries has been unsuccessful in biopanning experiments using microtiter plates. In the present study, by biopanning on LPS-conjugated epoxy beads, we repeatedly enriched clones encoding AWLPWAK and NLQEFLF. These peptides were found to interact with the polysaccharide moiety of LPS, which is highly variable among gram negative bacterial species. In addition, phages encoding these peptides preferentially bound to the LPS of Salmonella family. AWLPWAK-conjugated beads absorbed Salmonella enteritidis from solution and showed a preference for S. enteritidis over Escherichia coli. In summary, this study shows for the first time that a peptide screened from phage displays of combinatorial peptide libraries can be synthesized on beads and be used practically to concentrate bacterial cells from solution.

Original languageEnglish
Pages (from-to)312-317
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number1
DOIs
StatePublished - 1 Apr 2005

Keywords

  • Lipopolysaccharide
  • Peptide library
  • Phage display

Cite this

Kim, Y. G., Lee, C. S., Chung, W. J., Kim, E. M., Shin, D. S., Rhim, J. H., Lee, Y. S., Kim, B. G., & Chung, J. (2005). Screening of LPS-specific peptides from a phage display library using epoxy beads. Biochemical and Biophysical Research Communications, 329(1), 312-317. https://doi.org/10.1016/j.bbrc.2005.01.137