Retroviral vector expression in TCR transgenic CD4+ T cells

Youn Soo Choi, Shane Crotty

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The regulation of gene expression is key to understand the function of genes of interest. To explore the biological functions of genes, transgenic knock-in or knockout technologies have served as invaluable tools. While recent advances in molecular biology have introduced new techniques (i.e., CRISPR mediated gene editing) (Cong et al., Science 339(6121):819-823, 2013; Wang et al., Cell 153(4):910-918, 2013) for the generation of transgenic mice in a relatively short period of time, it can still take a long time to test biological hypotheses from scratch to design how to generate knock-in or knockout mice. Here, we describe methods to manipulate gene expression in T cell receptor (TCR) transgenic CD4 T cells, which allow us to investigate gene functions in the study of differentiation pathways of follicular helper T (Tfh) cells.

Original languageEnglish
Title of host publicationT Follicular Helper Cells
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages49-61
Number of pages13
ISBN (Electronic)9781493924981
ISBN (Print)9781493924974
DOIs
StatePublished - 2 Apr 2015

Fingerprint

T-cells
T-Cell Antigen Receptor
Gene Knock-In Techniques
Clustered Regularly Interspaced Short Palindromic Repeats
Genes
T-Lymphocytes
Gene Expression Regulation
Helper-Inducer T-Lymphocytes
Gene expression
Knockout Mice
Transgenic Mice
Molecular Biology
Technology
Gene Expression
Molecular biology
Gene Editing

Keywords

  • Knockdown
  • Overexpression
  • Primary CD4 T cells
  • Retroviral plasmid
  • TCR transgenic CD4 T cells
  • Tfh cells

Cite this

Choi, Y. S., & Crotty, S. (2015). Retroviral vector expression in TCR transgenic CD4+ T cells. In T Follicular Helper Cells: Methods and Protocols (pp. 49-61). Springer New York. https://doi.org/10.1007/978-1-4939-2498-1_5
Choi, Youn Soo ; Crotty, Shane. / Retroviral vector expression in TCR transgenic CD4+ T cells. T Follicular Helper Cells: Methods and Protocols. Springer New York, 2015. pp. 49-61
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Choi, YS & Crotty, S 2015, Retroviral vector expression in TCR transgenic CD4+ T cells. in T Follicular Helper Cells: Methods and Protocols. Springer New York, pp. 49-61. https://doi.org/10.1007/978-1-4939-2498-1_5

Retroviral vector expression in TCR transgenic CD4+ T cells. / Choi, Youn Soo; Crotty, Shane.

T Follicular Helper Cells: Methods and Protocols. Springer New York, 2015. p. 49-61.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Choi YS, Crotty S. Retroviral vector expression in TCR transgenic CD4+ T cells. In T Follicular Helper Cells: Methods and Protocols. Springer New York. 2015. p. 49-61 https://doi.org/10.1007/978-1-4939-2498-1_5