Requirement of MEF2D in the induced differentiation of HL60 promyeloid cells

Hyun Mu Shin, Ju Young Seoh, Hee Yong Chung, So Joung Choi, Myong Joon Hahn, Jae Seung Kang, Myung Sik Choi, Tae Hee Han

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The regulatory role of MEF2 (myocyte enhancer binding factor 2) proteins in nonmuscle tissues has not been well characterized. We examined the expression of MEF2 family members, namely, MEF2A, -B, -C, and -D, in the differentiation of HL60 promyeloid cells and observed the remarkable increase in the expressions of MEF2A and MEF2D proteins during the differentiation process into monocytes. To examine the role of MEF2, we expressed a dominant- negative form of MEF2D, without its transactivation domain, in HL60 cells. When the HL60 cell line expressing the mutant MEF2D was induced to differentiate by VitD3 treatment, cell surface expression of CD14 and the ability to reduce NBT, which are important characteristics of differentiated monocytes, were significantly decreased compared with control HL60 cells. These results show that MEF2D is required in the differentiation process along the monocyte/macrophage lineage, (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)1209-1214
Number of pages6
JournalMolecular Immunology
Volume36
Issue number18
DOIs
StatePublished - Dec 1999

Keywords

  • CD14
  • Differentiation
  • MEF2D
  • Monocytes/macrophages

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