Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice

Jae Hee Lee, Eun Ju Lee, Chae Hyun Lee, Jun Hong Park, Jae Yong Han, Jeong Mook Lim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: To evaluate the necessity of leukemia inhibitory factor (LIF) in establishing and self-renewing embryonic stem cells (ESCs). Design: Prospective animal model study. Setting: Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Korea. Animal(s): F1 hybrid B6D2F1 mice. Intervention(s): Inner cell mass (ICM) cells of blastocysts were cultured or commercially available ESCs were maintained in LIF-free or LIF-containing medium on mouse embryonic fibroblast (MEF) feeder. Main Outcome Measure(s): Cell morphology, LIF concentration, and mRNA expression. Result(s): The MEFs themselves secreted 146.5-175.3 pg/mL LIF in LIF-free medium. The ICM cells formed ESC-like colonies on MEF feeder, and E14 and R1 ESCs were successfully maintained in LIF-free medium. Expression of the genes either mediating LIF function or regulating stemness was not altered significantly, and change in the growth of ESCs was not prominent in LIF-free medium. Neither mRNA expression of differentiation-related genes nor differentiation into embryoid body was changed in the ESCs. Conclusion(s): Addition of LIF to culture medium is not necessary for establishing ICM-derived ESC-like colonies in the presence of fibroblast monolayer, and established ESCs can be maintained in an LIF-free medium.

Original languageEnglish
Pages (from-to)1133-1140
Number of pages8
JournalFertility and Sterility
Volume92
Issue number3
DOIs
StatePublished - 1 Sep 2009

Fingerprint

Leukemia Inhibitory Factor
Embryonic Stem Cells
Fibroblasts
Mouse Embryonic Stem Cells
Blastocyst Inner Cell Mass
Embryoid Bodies
Messenger RNA
Korea
Biotechnology
Germ Cells
Culture Media
Stem Cells
Animal Models
Outcome Assessment (Health Care)

Keywords

  • ESCs
  • LIF
  • Mouse
  • culture system
  • differentiation
  • stemness

Cite this

Lee, Jae Hee ; Lee, Eun Ju ; Lee, Chae Hyun ; Park, Jun Hong ; Han, Jae Yong ; Lim, Jeong Mook. / Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice. In: Fertility and Sterility. 2009 ; Vol. 92, No. 3. pp. 1133-1140.
@article{aebe05be18fe4611b092138e362c20a6,
title = "Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice",
abstract = "Objective: To evaluate the necessity of leukemia inhibitory factor (LIF) in establishing and self-renewing embryonic stem cells (ESCs). Design: Prospective animal model study. Setting: Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Korea. Animal(s): F1 hybrid B6D2F1 mice. Intervention(s): Inner cell mass (ICM) cells of blastocysts were cultured or commercially available ESCs were maintained in LIF-free or LIF-containing medium on mouse embryonic fibroblast (MEF) feeder. Main Outcome Measure(s): Cell morphology, LIF concentration, and mRNA expression. Result(s): The MEFs themselves secreted 146.5-175.3 pg/mL LIF in LIF-free medium. The ICM cells formed ESC-like colonies on MEF feeder, and E14 and R1 ESCs were successfully maintained in LIF-free medium. Expression of the genes either mediating LIF function or regulating stemness was not altered significantly, and change in the growth of ESCs was not prominent in LIF-free medium. Neither mRNA expression of differentiation-related genes nor differentiation into embryoid body was changed in the ESCs. Conclusion(s): Addition of LIF to culture medium is not necessary for establishing ICM-derived ESC-like colonies in the presence of fibroblast monolayer, and established ESCs can be maintained in an LIF-free medium.",
keywords = "ESCs, LIF, Mouse, culture system, differentiation, stemness",
author = "Lee, {Jae Hee} and Lee, {Eun Ju} and Lee, {Chae Hyun} and Park, {Jun Hong} and Han, {Jae Yong} and Lim, {Jeong Mook}",
year = "2009",
month = "9",
day = "1",
doi = "10.1016/j.fertnstert.2008.07.1733",
language = "English",
volume = "92",
pages = "1133--1140",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "3",

}

Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice. / Lee, Jae Hee; Lee, Eun Ju; Lee, Chae Hyun; Park, Jun Hong; Han, Jae Yong; Lim, Jeong Mook.

In: Fertility and Sterility, Vol. 92, No. 3, 01.09.2009, p. 1133-1140.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Requirement of leukemia inhibitory factor for establishing and maintaining embryonic stem cells in mice

AU - Lee, Jae Hee

AU - Lee, Eun Ju

AU - Lee, Chae Hyun

AU - Park, Jun Hong

AU - Han, Jae Yong

AU - Lim, Jeong Mook

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Objective: To evaluate the necessity of leukemia inhibitory factor (LIF) in establishing and self-renewing embryonic stem cells (ESCs). Design: Prospective animal model study. Setting: Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Korea. Animal(s): F1 hybrid B6D2F1 mice. Intervention(s): Inner cell mass (ICM) cells of blastocysts were cultured or commercially available ESCs were maintained in LIF-free or LIF-containing medium on mouse embryonic fibroblast (MEF) feeder. Main Outcome Measure(s): Cell morphology, LIF concentration, and mRNA expression. Result(s): The MEFs themselves secreted 146.5-175.3 pg/mL LIF in LIF-free medium. The ICM cells formed ESC-like colonies on MEF feeder, and E14 and R1 ESCs were successfully maintained in LIF-free medium. Expression of the genes either mediating LIF function or regulating stemness was not altered significantly, and change in the growth of ESCs was not prominent in LIF-free medium. Neither mRNA expression of differentiation-related genes nor differentiation into embryoid body was changed in the ESCs. Conclusion(s): Addition of LIF to culture medium is not necessary for establishing ICM-derived ESC-like colonies in the presence of fibroblast monolayer, and established ESCs can be maintained in an LIF-free medium.

AB - Objective: To evaluate the necessity of leukemia inhibitory factor (LIF) in establishing and self-renewing embryonic stem cells (ESCs). Design: Prospective animal model study. Setting: Gamete and Stem Cell Biotechnology Laboratory, Seoul National University, Korea. Animal(s): F1 hybrid B6D2F1 mice. Intervention(s): Inner cell mass (ICM) cells of blastocysts were cultured or commercially available ESCs were maintained in LIF-free or LIF-containing medium on mouse embryonic fibroblast (MEF) feeder. Main Outcome Measure(s): Cell morphology, LIF concentration, and mRNA expression. Result(s): The MEFs themselves secreted 146.5-175.3 pg/mL LIF in LIF-free medium. The ICM cells formed ESC-like colonies on MEF feeder, and E14 and R1 ESCs were successfully maintained in LIF-free medium. Expression of the genes either mediating LIF function or regulating stemness was not altered significantly, and change in the growth of ESCs was not prominent in LIF-free medium. Neither mRNA expression of differentiation-related genes nor differentiation into embryoid body was changed in the ESCs. Conclusion(s): Addition of LIF to culture medium is not necessary for establishing ICM-derived ESC-like colonies in the presence of fibroblast monolayer, and established ESCs can be maintained in an LIF-free medium.

KW - ESCs

KW - LIF

KW - Mouse

KW - culture system

KW - differentiation

KW - stemness

UR - http://www.scopus.com/inward/record.url?scp=69049107785&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2008.07.1733

DO - 10.1016/j.fertnstert.2008.07.1733

M3 - Article

C2 - 18829014

AN - SCOPUS:69049107785

VL - 92

SP - 1133

EP - 1140

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 3

ER -