Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity

Wolfgang Neuhofer, Seung Kyoon Woo, Ki Young Na, Rita Grünbein, Won Kun Park, Ohnn Nahm, Franz X. Beck, H. Moo Kwon

Research output: Contribution to journalArticleResearchpeer-review

39 Citations (Scopus)

Abstract

In response to ambient hypertonicity, TonEBP (tonicity-responsive enhancer binding protein) stimulates certain genes including those encoding cytokines, transporters for organic solutes, and a molecular chaperone. TonEBP is regulated in a bidirectional manner, upregulated by an increase in ambient tonicity while downregulated by a decrease. To investigate the role of intracellular ionic strength in the activity of TonEBP, we subjected Madin-Darby canine kidney cells to a variety of conditions. Electron microprobe analysis was performed to measure intracellular electrolytes. Under conditions in which changes in cell volume were similar, TonEBP activity correlated with the intracellular ionic strength regardless of the external tonicity. On the other hand, inhibition of the Na+/K+-ATPase and high external K+ concentration led to a decreased activity of TonEBP despite a marked increase in the intracellular ionic strength. Because isotonic swelling is known to occur under these conditions, these data suggest that dilution of the cytoplasmic constituents inhibits the activity of TonEBP. We conclude that intracellular ionic strength and water content are major factors that determine the activity of TonEBP.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number6 52-6
StatePublished - 1 Dec 2002

Fingerprint

NFATC Transcription Factors
Madin Darby Canine Kidney Cells
Ionic strength
Osmolar Concentration
Molecular Chaperones
Electron probe microanalysis
Cell Size
Water content
Electrolytes
Dilution
Swelling
Adenosine Triphosphatases
Down-Regulation
Genes
Electrons
Cytokines
Water

Keywords

  • Cell ionic strength
  • Cell volume
  • Heat shock protein 70
  • Madin-Darby canine kidney cells
  • Organic osmolytes

Cite this

Neuhofer, W., Woo, S. K., Na, K. Y., Grünbein, R., Park, W. K., Nahm, O., ... Moo Kwon, H. (2002). Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity. American Journal of Physiology - Cell Physiology, 283(6 52-6).
Neuhofer, Wolfgang ; Woo, Seung Kyoon ; Na, Ki Young ; Grünbein, Rita ; Park, Won Kun ; Nahm, Ohnn ; Beck, Franz X. ; Moo Kwon, H. / Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity. In: American Journal of Physiology - Cell Physiology. 2002 ; Vol. 283, No. 6 52-6.
@article{2256ff1dbeab429b901683025330d1a5,
title = "Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity",
abstract = "In response to ambient hypertonicity, TonEBP (tonicity-responsive enhancer binding protein) stimulates certain genes including those encoding cytokines, transporters for organic solutes, and a molecular chaperone. TonEBP is regulated in a bidirectional manner, upregulated by an increase in ambient tonicity while downregulated by a decrease. To investigate the role of intracellular ionic strength in the activity of TonEBP, we subjected Madin-Darby canine kidney cells to a variety of conditions. Electron microprobe analysis was performed to measure intracellular electrolytes. Under conditions in which changes in cell volume were similar, TonEBP activity correlated with the intracellular ionic strength regardless of the external tonicity. On the other hand, inhibition of the Na+/K+-ATPase and high external K+ concentration led to a decreased activity of TonEBP despite a marked increase in the intracellular ionic strength. Because isotonic swelling is known to occur under these conditions, these data suggest that dilution of the cytoplasmic constituents inhibits the activity of TonEBP. We conclude that intracellular ionic strength and water content are major factors that determine the activity of TonEBP.",
keywords = "Cell ionic strength, Cell volume, Heat shock protein 70, Madin-Darby canine kidney cells, Organic osmolytes",
author = "Wolfgang Neuhofer and Woo, {Seung Kyoon} and Na, {Ki Young} and Rita Gr{\"u}nbein and Park, {Won Kun} and Ohnn Nahm and Beck, {Franz X.} and {Moo Kwon}, H.",
year = "2002",
month = "12",
day = "1",
language = "English",
volume = "283",
journal = "American Journal of Physiology - Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "6 52-6",

}

Neuhofer, W, Woo, SK, Na, KY, Grünbein, R, Park, WK, Nahm, O, Beck, FX & Moo Kwon, H 2002, 'Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity', American Journal of Physiology - Cell Physiology, vol. 283, no. 6 52-6.

Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity. / Neuhofer, Wolfgang; Woo, Seung Kyoon; Na, Ki Young; Grünbein, Rita; Park, Won Kun; Nahm, Ohnn; Beck, Franz X.; Moo Kwon, H.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 6 52-6, 01.12.2002.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity

AU - Neuhofer, Wolfgang

AU - Woo, Seung Kyoon

AU - Na, Ki Young

AU - Grünbein, Rita

AU - Park, Won Kun

AU - Nahm, Ohnn

AU - Beck, Franz X.

AU - Moo Kwon, H.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - In response to ambient hypertonicity, TonEBP (tonicity-responsive enhancer binding protein) stimulates certain genes including those encoding cytokines, transporters for organic solutes, and a molecular chaperone. TonEBP is regulated in a bidirectional manner, upregulated by an increase in ambient tonicity while downregulated by a decrease. To investigate the role of intracellular ionic strength in the activity of TonEBP, we subjected Madin-Darby canine kidney cells to a variety of conditions. Electron microprobe analysis was performed to measure intracellular electrolytes. Under conditions in which changes in cell volume were similar, TonEBP activity correlated with the intracellular ionic strength regardless of the external tonicity. On the other hand, inhibition of the Na+/K+-ATPase and high external K+ concentration led to a decreased activity of TonEBP despite a marked increase in the intracellular ionic strength. Because isotonic swelling is known to occur under these conditions, these data suggest that dilution of the cytoplasmic constituents inhibits the activity of TonEBP. We conclude that intracellular ionic strength and water content are major factors that determine the activity of TonEBP.

AB - In response to ambient hypertonicity, TonEBP (tonicity-responsive enhancer binding protein) stimulates certain genes including those encoding cytokines, transporters for organic solutes, and a molecular chaperone. TonEBP is regulated in a bidirectional manner, upregulated by an increase in ambient tonicity while downregulated by a decrease. To investigate the role of intracellular ionic strength in the activity of TonEBP, we subjected Madin-Darby canine kidney cells to a variety of conditions. Electron microprobe analysis was performed to measure intracellular electrolytes. Under conditions in which changes in cell volume were similar, TonEBP activity correlated with the intracellular ionic strength regardless of the external tonicity. On the other hand, inhibition of the Na+/K+-ATPase and high external K+ concentration led to a decreased activity of TonEBP despite a marked increase in the intracellular ionic strength. Because isotonic swelling is known to occur under these conditions, these data suggest that dilution of the cytoplasmic constituents inhibits the activity of TonEBP. We conclude that intracellular ionic strength and water content are major factors that determine the activity of TonEBP.

KW - Cell ionic strength

KW - Cell volume

KW - Heat shock protein 70

KW - Madin-Darby canine kidney cells

KW - Organic osmolytes

UR - http://www.scopus.com/inward/record.url?scp=0036889003&partnerID=8YFLogxK

M3 - Article

VL - 283

JO - American Journal of Physiology - Cell Physiology

JF - American Journal of Physiology - Cell Physiology

SN - 0363-6143

IS - 6 52-6

ER -