Real-time light-guided vocal fold injection: An in vivo feasibility study in a canine model

Hee Young Son, Sanghoon Kim, Ramla Talib Mohammad, Gene Huh, Hyojin Kim, Woo Jin Jeong, Wonjae Cha

Research output: Contribution to journalArticlepeer-review


Objectives. The transcutaneous approach is a good option for office-based vocal fold injection (VFI). However, precise localization requires extensive experience because the needle tip is invisible in small and complex laryngeal spaces. Recently, real-time light-guided VFI (RL-VFI) was proposed as a new technique that allows simultaneous injection under precise needle localization by light guidance. Herein, we aimed to verify the feasibility of RL-VFI in an in vivo canine model and explored its clinical usefulness. Methods. The device for RL-VFI comprised a light source (light-emitting diode modules [10 W] of red color [650 nm]) and injectors (1.5 inches, 23 gauge). An adult male beagle was used for the experiment. After tracheostomy, a rigid laryn-goscope was inserted and suspended to expose the larynx. A flexible naso-laryngoscopy system was used to visualize the vocal folds. Results. RL-VFI was performed using various transcutaneous approaches, including the cricothyroid, transthyroid, and transhyoid approaches. Light guidance helped identify the path of the needle and prevent inadvertent penetration. The location of the needle tip was accurately indicated by the light. The illuminated needle could be easily placed at the intended points in the vocal fold with real-time visual-motor feedback. Hyaluronic acid could be simultaneously injected lateral to the vocal process under light guidance without manipulation of the device. Conclusion. RL-VFI was found to be safe and feasible in an in vivo canine model, providing precise localization and visual-motor feedback. The clinical application of RL-VFI is expected to improve the safety and precision of VFI.

Original languageEnglish
Pages (from-to)338-346
Number of pages9
JournalClinical and Experimental Otorhinolaryngology
Issue number3
StatePublished - Aug 2021


  • In Vivo Animal Study
  • Real-Time Light-Guided Vocal Fold Injection
  • Transcutaneous Approach
  • Vocal Fold Injection
  • Vocal Fold Palsy


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