Rapid, multiplexed, and nucleic acid amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor

Leila Kashefi-Kheyrabadi, Huynh Vu Nguyen, Anna Go, Changyoon Baek, Nayoon Jang, Jiwon Michelle Lee, Nam Hyuk Cho, Junhong Min, Min Ho Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Considering the worldwide health crisis associated with highly contagious severe respiratory disease of COVID-19 outbreak, the development of multiplexed, simple and rapid diagnostic platforms to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is in high demand. Here, a nucleic acid amplification-free electrochemical biosensor based on four-way junction (4-WJ) hybridization is presented for the detection of SARS-CoV-2. To form a 4-WJ structure, a Universal DNA-Hairpin (UDH) probe is hybridized with two adaptor strands and a SARS-CoV-2 RNA target. One of the adaptor strands is functionalized with a redox mediator that can be detected using an electrochemical biosensor. The biosensor could simultaneously detect 5.0 and 6.8 ag/μL of S and Orf1ab genes, respectively, within 1 h. The biosensor was evaluated with 21 clinical samples (16 positive and 5 negative). The results revealed a satisfactory agreement with qRT-PCR. In conclusion, this biosensor has the potential to be used as an on-site, real-time diagnostic test for COVID-19.

Original languageEnglish
Article number113649
JournalBiosensors and Bioelectronics
Volume195
DOIs
StatePublished - 1 Jan 2022

Keywords

  • COVID-19
  • Electrochemical biosensor
  • Orf1ab gene
  • S gene
  • SARS-CoV-2 RNA

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