Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress

Kyoung Ah Kang, In Kyung Lee, Rui Zhang, Mei Jing Piao, Ki Cheon Kim, Sang Young Kim, Taekyun Shin, Bum Joon Kim, Nam Ho Lee, Jin Won Hyun

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The radioprotective effect of geraniin, a tannin compound isolated from Nymphaea tetragona Georgi var. (Nymphaeaceae), against γ-radiation-induced damage was investigated in Chinese hamster lung fibroblast (V79-4) cells. Geraniin recovered cell viability detected by MTT test and colony formation assay, which was compromised by γ-radiation, and reduced the γ-radiation-induced apoptosis by the inhibition of loss of the mitochondrial membrane potential. Geraniin protected cellular components (lipid membrane, cellular protein, and DNA) damaged by γ-radiation, which was detected by lipid peroxidation, protein carbonyl formation, and comet assay. Geraniin significantly reduced the level of intracellular reactive oxygen species generated by γ-radiation, which was detected using spectrofluorometer, flow cytometer, and confocal microscope after 2′,7′-dichlorodihydrofluorescein diacetate staining. Geraniin normalized the superoxide dismutase and catalase activities, which were decreased by γ-radiation. These results suggest that geraniin protects cells against radiation-induced oxidative stress via enhancing of antioxidant enzyme activities and attenuating of cellular damage.

Original languageEnglish
Pages (from-to)83-94
Number of pages12
JournalCell Biology and Toxicology
Volume27
Issue number2
DOIs
StatePublished - 1 Apr 2011

Fingerprint

Oxidative stress
Oxidative Stress
Radiation
Apoptosis
Nymphaeaceae
Assays
Nymphaea
Protein Carbonylation
Comet Assay
Mitochondrial Membrane Potential
Tannins
Enzyme activity
Fibroblasts
Membrane Lipids
Cricetulus
Geraniin
Catalase
Lipid Peroxidation
Superoxide Dismutase
Reactive Oxygen Species

Keywords

  • Apoptosis
  • Geraniin
  • Radioprotective effect
  • Reactive oxygen species
  • γ-Radiation

Cite this

Kang, Kyoung Ah ; Lee, In Kyung ; Zhang, Rui ; Piao, Mei Jing ; Kim, Ki Cheon ; Kim, Sang Young ; Shin, Taekyun ; Kim, Bum Joon ; Lee, Nam Ho ; Hyun, Jin Won. / Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress. In: Cell Biology and Toxicology. 2011 ; Vol. 27, No. 2. pp. 83-94.
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Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress. / Kang, Kyoung Ah; Lee, In Kyung; Zhang, Rui; Piao, Mei Jing; Kim, Ki Cheon; Kim, Sang Young; Shin, Taekyun; Kim, Bum Joon; Lee, Nam Ho; Hyun, Jin Won.

In: Cell Biology and Toxicology, Vol. 27, No. 2, 01.04.2011, p. 83-94.

Research output: Contribution to journalArticle

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