Quantum-dots-based detection of hepatitis C virus (HCV) NS3 using RNA aptamer on chip

Changhyun Roh, Ho Young Lee, Sang Eun Kim, Sung Kee Jo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

BACKGROUND: Over 170 million people, more than 3% of the world's population, suffer from the hepatitis C virus (HCV) infection and the rate of death from liver-related mortality to HCV has increased. In respect of this, the development of assays for biological imaging should be urgently considered as an essential factor in diagnosis. RESULTS: A novel HCV-detecting technique using a nanoparticle-supported aptamer probe was demonstrated. With the aid of nanoparticle quantum dots (QDs) with carboxyl group as an imaging probe, and 5′-end-amine-modified RNA oligonucleotide as a capturing probe, target HCV NS3 was visually detected on chip. The QDs-based RNA aptamer for HCV NS3 showed high selectivity and specificity against other protein such as BSA. The detection limit of HCV NS3 protein was 5 ng mL-1 level. CONCLUSION: With a novel strategy for protein-aptamer interaction, the feasibility of applying QDs-based fluorescent detection technique to HCV viral protein assay for the development of a protein biochipwas demonstrated. This scheme of QDs-mediated imaging with a target-oriented specific RNA aptamer for the detection of infectious HCV diseases provides an efficient strategy and a promising new platform for monitoring applications.

Original languageEnglish
Pages (from-to)1130-1134
Number of pages5
JournalJournal of Chemical Technology and Biotechnology
Volume85
Issue number8
DOIs
StatePublished - Aug 2010

Keywords

  • Diagnosis
  • Hepatitis C virus (HCV)
  • Imaging
  • Nanoparticle
  • Quantum dots (QDs)
  • RNA aptamer

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