Quantum-dots-based detection of hepatitis C virus (HCV) NS3 using RNA aptamer on chip

Changhyun Roh, Ho Young Lee, Sang Eun Kim, Sung Kee Jo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

BACKGROUND: Over 170 million people, more than 3% of the world's population, suffer from the hepatitis C virus (HCV) infection and the rate of death from liver-related mortality to HCV has increased. In respect of this, the development of assays for biological imaging should be urgently considered as an essential factor in diagnosis. RESULTS: A novel HCV-detecting technique using a nanoparticle-supported aptamer probe was demonstrated. With the aid of nanoparticle quantum dots (QDs) with carboxyl group as an imaging probe, and 5′-end-amine-modified RNA oligonucleotide as a capturing probe, target HCV NS3 was visually detected on chip. The QDs-based RNA aptamer for HCV NS3 showed high selectivity and specificity against other protein such as BSA. The detection limit of HCV NS3 protein was 5 ng mL-1 level. CONCLUSION: With a novel strategy for protein-aptamer interaction, the feasibility of applying QDs-based fluorescent detection technique to HCV viral protein assay for the development of a protein biochipwas demonstrated. This scheme of QDs-mediated imaging with a target-oriented specific RNA aptamer for the detection of infectious HCV diseases provides an efficient strategy and a promising new platform for monitoring applications.

Original languageEnglish
Pages (from-to)1130-1134
Number of pages5
JournalJournal of Chemical Technology and Biotechnology
Volume85
Issue number8
DOIs
StatePublished - 1 Aug 2010

Fingerprint

Nucleotide Aptamers
Quantum Dots
hepatitis
RNA
Viruses
Hepacivirus
Semiconductor quantum dots
virus
Proteins
protein
Virus Diseases
Imaging techniques
probe
Nanoparticles
Assays
Hepatovirus
assay
Mortality
Viral Proteins
detection

Keywords

  • Diagnosis
  • Hepatitis C virus (HCV)
  • Imaging
  • Nanoparticle
  • Quantum dots (QDs)
  • RNA aptamer

Cite this

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abstract = "BACKGROUND: Over 170 million people, more than 3{\%} of the world's population, suffer from the hepatitis C virus (HCV) infection and the rate of death from liver-related mortality to HCV has increased. In respect of this, the development of assays for biological imaging should be urgently considered as an essential factor in diagnosis. RESULTS: A novel HCV-detecting technique using a nanoparticle-supported aptamer probe was demonstrated. With the aid of nanoparticle quantum dots (QDs) with carboxyl group as an imaging probe, and 5′-end-amine-modified RNA oligonucleotide as a capturing probe, target HCV NS3 was visually detected on chip. The QDs-based RNA aptamer for HCV NS3 showed high selectivity and specificity against other protein such as BSA. The detection limit of HCV NS3 protein was 5 ng mL-1 level. CONCLUSION: With a novel strategy for protein-aptamer interaction, the feasibility of applying QDs-based fluorescent detection technique to HCV viral protein assay for the development of a protein biochipwas demonstrated. This scheme of QDs-mediated imaging with a target-oriented specific RNA aptamer for the detection of infectious HCV diseases provides an efficient strategy and a promising new platform for monitoring applications.",
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Quantum-dots-based detection of hepatitis C virus (HCV) NS3 using RNA aptamer on chip. / Roh, Changhyun; Lee, Ho Young; Kim, Sang Eun; Jo, Sung Kee.

In: Journal of Chemical Technology and Biotechnology, Vol. 85, No. 8, 01.08.2010, p. 1130-1134.

Research output: Contribution to journalArticle

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