Protective role of kallistatin in oxygen-glucose deprivation and reoxygenation in human umbilical vein endothelial cells

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Abstract

Objective Ischemia-reperfusion (IR) injury is implicated in various clinical diseases. Kallistatin attenuates oxidative stress, and its deficiency has been associated with poor neurological outcomes after cardiac arrest. The present study investigated the antioxidant mechanism through which kallistatin prevents IR injury. Methods Human umbilical vein endothelial cells (HUVECs) were transfected with small interfering RNA (siRNA) targeting the human kallistatin gene (SERPINA4). Following SERPINA4 knockdown, the level of kallistatin expression was measured. To induce IR injury, HUVECs were exposed to 24 h of oxygen-glucose deprivation and reoxygenation (OGD/R). To evaluate the effect of SERPINA4 knockdown on OGD/R, cell viability and the concentration of kallistatin, endothelial nitric oxide synthase (eNOS) and total NO were measured. Results SERPINA4 siRNA transfection suppressed the expression of kallistatin in HUVECs. Exposure to OGD/R reduced cell viability, and this effect was more pronounced in SERPINA4 knockdown cells compared with controls. SERPINA4 knockdown significantly reduced kallistatin concentration regardless of OGD/R, with a more pronounced effect observed without OGD/R. Furthermore, SERPINA4 knockdown significantly decreased eNOS concentrations induced by OGD/R (P<0.01) but did not significantly affect the change in total NO concentration (P=0.728). Conclusion The knockdown of SERPINA4 resulted in increased vulnerability of HUVECs to OGD/ R and significantly affected the change in eNOS level induced by OGD/R. These findings suggest that the protective effect of kallistatin against IR injury may contribute to its eNOS-promoting effect.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalClinical and Experimental Emergency Medicine
Volume11
Issue number1
DOIs
StatePublished - Mar 2024

Bibliographical note

Publisher Copyright:
© 2024 The Korean Society of Emergency Medicine.

Keywords

  • Heart arrest
  • Nitric oxide
  • Nitric oxide synthase
  • Reactive oxygen species
  • Reperfusion injury

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