Prevalence of anti-β2 glycoprotein-I antibody in patients with primary or secondary immune thrombocytopenia

Kyung Soon Song, Young Ah Kim, Hyon Suk Kim, Jae Gyun Lim, Hyun Kyung Kim, Kwang Il Park, Quehn Park

Research output: Contribution to journalArticle

Abstract

We determined the prevalence of antibodies to β2 glycoprotein-I (β2GPI) in sera of 57 patients with primary or secondary immune thrombocytopenia positive for platelet antibody (PAIgG) and in 16 healthy control subjects. Both isotypes (IgG and IgM) of anti-β2GPI were detected by means of semiquantitative ELISA and platelet antibodies were measured by means of flow cytometry using FITC-labeled anti-human IgG. Six (10.5%) of 57 patients with immune thrombocytopenia had anti-β2GPI, whereas none of the 16 healthy subjects had anti-β2GPI. Four were positive for IgM anti-β2GPI, one for IgG anti-β2GPI, and one was positive both for IgM and IgG anti-β2GPI. There was no significant association between the presence of anti-β2GPI and the severity of thrombocytopenia. Our findings suggest that some patients with immune thrombocytopenia have anti-β2GPI in addition to the increased PAIgG; however, the results do not support a major role for anti-β2GPI in the pathogenesis of immune thrombocytopenia.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalPlatelets
Volume10
Issue number4
DOIs
StatePublished - 28 Aug 1999
Externally publishedYes

Fingerprint

Idiopathic Thrombocytopenic Purpura
Glycoproteins
Antibodies
Healthy Volunteers
Blood Platelets
Fluorescein-5-isothiocyanate
Thrombocytopenia
Flow Cytometry
Enzyme-Linked Immunosorbent Assay

Cite this

Song, Kyung Soon ; Kim, Young Ah ; Kim, Hyon Suk ; Lim, Jae Gyun ; Kim, Hyun Kyung ; Park, Kwang Il ; Park, Quehn. / Prevalence of anti-β2 glycoprotein-I antibody in patients with primary or secondary immune thrombocytopenia. In: Platelets. 1999 ; Vol. 10, No. 4. pp. 219-222.
@article{8137fd54cfe244cca275207165ee7f5a,
title = "Prevalence of anti-β2 glycoprotein-I antibody in patients with primary or secondary immune thrombocytopenia",
abstract = "We determined the prevalence of antibodies to β2 glycoprotein-I (β2GPI) in sera of 57 patients with primary or secondary immune thrombocytopenia positive for platelet antibody (PAIgG) and in 16 healthy control subjects. Both isotypes (IgG and IgM) of anti-β2GPI were detected by means of semiquantitative ELISA and platelet antibodies were measured by means of flow cytometry using FITC-labeled anti-human IgG. Six (10.5{\%}) of 57 patients with immune thrombocytopenia had anti-β2GPI, whereas none of the 16 healthy subjects had anti-β2GPI. Four were positive for IgM anti-β2GPI, one for IgG anti-β2GPI, and one was positive both for IgM and IgG anti-β2GPI. There was no significant association between the presence of anti-β2GPI and the severity of thrombocytopenia. Our findings suggest that some patients with immune thrombocytopenia have anti-β2GPI in addition to the increased PAIgG; however, the results do not support a major role for anti-β2GPI in the pathogenesis of immune thrombocytopenia.",
author = "Song, {Kyung Soon} and Kim, {Young Ah} and Kim, {Hyon Suk} and Lim, {Jae Gyun} and Kim, {Hyun Kyung} and Park, {Kwang Il} and Quehn Park",
year = "1999",
month = "8",
day = "28",
doi = "10.1080/09537109976059",
language = "English",
volume = "10",
pages = "219--222",
journal = "Platelets",
issn = "0953-7104",
publisher = "Informa Healthcare",
number = "4",

}

Prevalence of anti-β2 glycoprotein-I antibody in patients with primary or secondary immune thrombocytopenia. / Song, Kyung Soon; Kim, Young Ah; Kim, Hyon Suk; Lim, Jae Gyun; Kim, Hyun Kyung; Park, Kwang Il; Park, Quehn.

In: Platelets, Vol. 10, No. 4, 28.08.1999, p. 219-222.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prevalence of anti-β2 glycoprotein-I antibody in patients with primary or secondary immune thrombocytopenia

AU - Song, Kyung Soon

AU - Kim, Young Ah

AU - Kim, Hyon Suk

AU - Lim, Jae Gyun

AU - Kim, Hyun Kyung

AU - Park, Kwang Il

AU - Park, Quehn

PY - 1999/8/28

Y1 - 1999/8/28

N2 - We determined the prevalence of antibodies to β2 glycoprotein-I (β2GPI) in sera of 57 patients with primary or secondary immune thrombocytopenia positive for platelet antibody (PAIgG) and in 16 healthy control subjects. Both isotypes (IgG and IgM) of anti-β2GPI were detected by means of semiquantitative ELISA and platelet antibodies were measured by means of flow cytometry using FITC-labeled anti-human IgG. Six (10.5%) of 57 patients with immune thrombocytopenia had anti-β2GPI, whereas none of the 16 healthy subjects had anti-β2GPI. Four were positive for IgM anti-β2GPI, one for IgG anti-β2GPI, and one was positive both for IgM and IgG anti-β2GPI. There was no significant association between the presence of anti-β2GPI and the severity of thrombocytopenia. Our findings suggest that some patients with immune thrombocytopenia have anti-β2GPI in addition to the increased PAIgG; however, the results do not support a major role for anti-β2GPI in the pathogenesis of immune thrombocytopenia.

AB - We determined the prevalence of antibodies to β2 glycoprotein-I (β2GPI) in sera of 57 patients with primary or secondary immune thrombocytopenia positive for platelet antibody (PAIgG) and in 16 healthy control subjects. Both isotypes (IgG and IgM) of anti-β2GPI were detected by means of semiquantitative ELISA and platelet antibodies were measured by means of flow cytometry using FITC-labeled anti-human IgG. Six (10.5%) of 57 patients with immune thrombocytopenia had anti-β2GPI, whereas none of the 16 healthy subjects had anti-β2GPI. Four were positive for IgM anti-β2GPI, one for IgG anti-β2GPI, and one was positive both for IgM and IgG anti-β2GPI. There was no significant association between the presence of anti-β2GPI and the severity of thrombocytopenia. Our findings suggest that some patients with immune thrombocytopenia have anti-β2GPI in addition to the increased PAIgG; however, the results do not support a major role for anti-β2GPI in the pathogenesis of immune thrombocytopenia.

UR - http://www.scopus.com/inward/record.url?scp=0032798595&partnerID=8YFLogxK

U2 - 10.1080/09537109976059

DO - 10.1080/09537109976059

M3 - Article

C2 - 16801095

AN - SCOPUS:0032798595

VL - 10

SP - 219

EP - 222

JO - Platelets

JF - Platelets

SN - 0953-7104

IS - 4

ER -