Population structure of the Bacillus cereus group as determined by sequence analysis of six housekeeping genes and the plcR gene

Kwan Soo Ko, Jong Wan Kim, Jong Man Kim, Wonyong Kim, Sang In Chung, Ik Jung Kim, Yoon Hoh Kook

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The population structure of the Bacillus cereus group (52 strains of B. anthracis, B. cereus, and B. thuringiensis) was investigated by sequencing seven gene fragments (rpoB,gyrB,pycA, mdh, mbl, mutS, and plcR). Most of the strains were classifiable into two large subgroups in six housekeeping gene trees but not in the plcR tree. In addition, several consistent clusters were identified, which were unrelated to species distinction. Moreover, interrelationships among these clusters were incongruent in each gene tree. The incongruence length difference test and split decomposition analyses also showed incongruences between genes, suggesting horizontal gene transfer. The plcR gene was observed to have characteristics that differed from those of the other genes in terms of phylogenetic topology and pattern of sequence diversity. Thus, we suggest that the evolutionary history of the PlcR regulon differs from those of the other chromosomal genes and that recombination of the plcR gene may be frequent. The homogeneity of B. anthracis, which is depicted as an independent lineage in phylogenetic trees, is suggested to be of recent origin or to be due to the narrow taxonomic definition of species.

Original languageEnglish
Pages (from-to)5253-5261
Number of pages9
JournalInfection and Immunity
Volume72
Issue number9
DOIs
StatePublished - 1 Sep 2004

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Bacillus cereus
Essential Genes
Sequence Analysis
Population
Genes
Regulon
Horizontal Gene Transfer
Genetic Recombination
History

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Ko, Kwan Soo ; Kim, Jong Wan ; Kim, Jong Man ; Kim, Wonyong ; Chung, Sang In ; Kim, Ik Jung ; Kook, Yoon Hoh. / Population structure of the Bacillus cereus group as determined by sequence analysis of six housekeeping genes and the plcR gene. In: Infection and Immunity. 2004 ; Vol. 72, No. 9. pp. 5253-5261.
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Population structure of the Bacillus cereus group as determined by sequence analysis of six housekeeping genes and the plcR gene. / Ko, Kwan Soo; Kim, Jong Wan; Kim, Jong Man; Kim, Wonyong; Chung, Sang In; Kim, Ik Jung; Kook, Yoon Hoh.

In: Infection and Immunity, Vol. 72, No. 9, 01.09.2004, p. 5253-5261.

Research output: Contribution to journalArticle

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