Polymorphisms of ADIPOR1 and ADIPOR2 are associated with phenotypes of type 2 diabetes in Koreans

Jin Taek Kim, Yeonjung Kim, Young Min Cho, Bo Kyung Koo, Eun Kyung Lee, Hyoung Doo Shin, Hak Chul Jang, Jung Won Choi, Bermseok Oh, Kyong Soo Park

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Abstract

Objective: Adiponectin receptors 1 and 2 (ADIPOR1 and ADIPOR2) are considered as candidate genes for type 2 diabetes because they mediate the metabolic effects of adiponectin on target tissues. We investigated whether common polymorphisms of ADIPOR1 and ADIPOR2 are associated with type 2 diabetes or its related phenotypes in Koreans. Design and patients: By sequencing of the DNA samples from 24 unrelated Korean subjects, we selected seven single nucleotide polymorphisms (SNPs) from ADIPOR1 and four SNPs from ADIPOR2 for genotyping in 757 type 2 diabetic patients and 644 nondiabetic subjects. Results: None of the SNPs were associated with the risk of type 2 diabetes. However, g.-7309A>G (rs75172865) in ADIPOR1 was associated with lower insulin resistance, measured by the homeostasis model assessment of insulin resistance (HOMA-IR). Thereafter, we transfected three different human cell lines with plasmids harbouring g.-7309A>G and found that this variant reduced the promoter activity of ADIPOR1. In ADIPOR2, g.-63442G (novel SNP) and g.33447T (rs1044471) were associated with smaller waist circumference. Conclusions: None of the SNPs in either ADIPOR1 or ADIPOR2 were associated with the risk of type 2 diabetes in Koreans. However, the altered activity of the ADIPOR1 promoter was associated with insulin resistance and SNPs of ADIPOR2 were associated with waist circumference.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalClinical Endocrinology
Volume70
Issue number1
DOIs
StatePublished - 1 Jan 2009

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Type 2 Diabetes Mellitus
Single Nucleotide Polymorphism
Phenotype
Insulin Resistance
Waist Circumference
Adiponectin Receptors
Adiponectin
DNA Sequence Analysis
Plasmids
Homeostasis
Cell Line
Genes

Cite this

Kim, Jin Taek ; Kim, Yeonjung ; Cho, Young Min ; Koo, Bo Kyung ; Lee, Eun Kyung ; Shin, Hyoung Doo ; Jang, Hak Chul ; Choi, Jung Won ; Oh, Bermseok ; Park, Kyong Soo. / Polymorphisms of ADIPOR1 and ADIPOR2 are associated with phenotypes of type 2 diabetes in Koreans. In: Clinical Endocrinology. 2009 ; Vol. 70, No. 1. pp. 66-74.
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Polymorphisms of ADIPOR1 and ADIPOR2 are associated with phenotypes of type 2 diabetes in Koreans. / Kim, Jin Taek; Kim, Yeonjung; Cho, Young Min; Koo, Bo Kyung; Lee, Eun Kyung; Shin, Hyoung Doo; Jang, Hak Chul; Choi, Jung Won; Oh, Bermseok; Park, Kyong Soo.

In: Clinical Endocrinology, Vol. 70, No. 1, 01.01.2009, p. 66-74.

Research output: Contribution to journalArticle

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T1 - Polymorphisms of ADIPOR1 and ADIPOR2 are associated with phenotypes of type 2 diabetes in Koreans

AU - Kim, Jin Taek

AU - Kim, Yeonjung

AU - Cho, Young Min

AU - Koo, Bo Kyung

AU - Lee, Eun Kyung

AU - Shin, Hyoung Doo

AU - Jang, Hak Chul

AU - Choi, Jung Won

AU - Oh, Bermseok

AU - Park, Kyong Soo

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Y1 - 2009/1/1

N2 - Objective: Adiponectin receptors 1 and 2 (ADIPOR1 and ADIPOR2) are considered as candidate genes for type 2 diabetes because they mediate the metabolic effects of adiponectin on target tissues. We investigated whether common polymorphisms of ADIPOR1 and ADIPOR2 are associated with type 2 diabetes or its related phenotypes in Koreans. Design and patients: By sequencing of the DNA samples from 24 unrelated Korean subjects, we selected seven single nucleotide polymorphisms (SNPs) from ADIPOR1 and four SNPs from ADIPOR2 for genotyping in 757 type 2 diabetic patients and 644 nondiabetic subjects. Results: None of the SNPs were associated with the risk of type 2 diabetes. However, g.-7309A>G (rs75172865) in ADIPOR1 was associated with lower insulin resistance, measured by the homeostasis model assessment of insulin resistance (HOMA-IR). Thereafter, we transfected three different human cell lines with plasmids harbouring g.-7309A>G and found that this variant reduced the promoter activity of ADIPOR1. In ADIPOR2, g.-63442G (novel SNP) and g.33447T (rs1044471) were associated with smaller waist circumference. Conclusions: None of the SNPs in either ADIPOR1 or ADIPOR2 were associated with the risk of type 2 diabetes in Koreans. However, the altered activity of the ADIPOR1 promoter was associated with insulin resistance and SNPs of ADIPOR2 were associated with waist circumference.

AB - Objective: Adiponectin receptors 1 and 2 (ADIPOR1 and ADIPOR2) are considered as candidate genes for type 2 diabetes because they mediate the metabolic effects of adiponectin on target tissues. We investigated whether common polymorphisms of ADIPOR1 and ADIPOR2 are associated with type 2 diabetes or its related phenotypes in Koreans. Design and patients: By sequencing of the DNA samples from 24 unrelated Korean subjects, we selected seven single nucleotide polymorphisms (SNPs) from ADIPOR1 and four SNPs from ADIPOR2 for genotyping in 757 type 2 diabetic patients and 644 nondiabetic subjects. Results: None of the SNPs were associated with the risk of type 2 diabetes. However, g.-7309A>G (rs75172865) in ADIPOR1 was associated with lower insulin resistance, measured by the homeostasis model assessment of insulin resistance (HOMA-IR). Thereafter, we transfected three different human cell lines with plasmids harbouring g.-7309A>G and found that this variant reduced the promoter activity of ADIPOR1. In ADIPOR2, g.-63442G (novel SNP) and g.33447T (rs1044471) were associated with smaller waist circumference. Conclusions: None of the SNPs in either ADIPOR1 or ADIPOR2 were associated with the risk of type 2 diabetes in Koreans. However, the altered activity of the ADIPOR1 promoter was associated with insulin resistance and SNPs of ADIPOR2 were associated with waist circumference.

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