Plasma E-selectin and kallistatin as predictive markers of histologic chorioamnionitis in women with preterm premature rupture of membranes

Iseop Cho, Kyong No Lee, Eunwook Joo, Yu Mi Kim, Tae Eun Kim, Kyo Hoon Park

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Problem: We aimed to assess the predictive potential of 12 plasma biomarkers to predict acute histologic chorioamnionitis (HCA) in women with preterm premature rupture of membranes (PPROM) and to develop multi-biomarker panels based on these biomarkers in combination with widely used conventional laboratory markers. Method of study: This was a retrospective cohort study involving 81 singleton pregnant women (24–34 weeks of gestation) who delivered within 96 h of blood sampling. White blood cell (WBC) count, differential counts, and C-reactive protein (CRP) levels were measured at admission. The levels of DKK-3, Fas, haptoglobin, IGFBP-2, kallistatin, MIP-1α, MMP-2, MMP-8, pentraxin 3, progranulin, E-selectin, and P-selectin were evaluated by ELISA using stored plasma samples. The primary outcome measure was acute HCA. Results: Multivariate analyses showed that low plasma E-selectin and kallistatin levels were independently associated with HCA occurrence after adjusting for gestational age. Using a stepwise regression analysis, a multi-biomarker panel comprising plasma E-selectin, serum CRP, and WBC was developed, which provided a good prediction of acute HCA in women with PPROM (area under the curve [AUC], 0.899), with a significantly higher AUC than that of any single variable included in the panel (P < 0.05). The plasma levels of DKK-3, Fas, haptoglobin, IGFBP-2, MIP-1α, MMP-2, MMP-8, pentraxin 3, and P-selectin were not significantly associated with HCA occurrence. Conclusions: This study identified E-selectin and kallistatin as potential plasma biomarkers associated with acute HCA in women with PPROM. Their combined analysis with serum CRP and WBC counts significantly improved acute HCA diagnosis.

Original languageEnglish
Article numbere13584
JournalAmerican Journal of Reproductive Immunology
Volume88
Issue number3
DOIs
StatePublished - Sep 2022
Externally publishedYes

Keywords

  • E-selectin
  • biomarker
  • histologic chorioamnionitis
  • kallistatin
  • multi-biomarker panel
  • plasma
  • preterm premature rupture of membranes

Fingerprint

Dive into the research topics of 'Plasma E-selectin and kallistatin as predictive markers of histologic chorioamnionitis in women with preterm premature rupture of membranes'. Together they form a unique fingerprint.

Cite this