Phenotypic and metabolic characteristics of maternal monocytes and granulocytes in preterm labor with intact membranes

Maria Teresa Gervasi, Tinnakorn Chaiworapongsa, Nihal Naccasha, Sean Blackwell, Bohyun Yoon, Eli Maymon, Roberto Romero

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

OBJECTIVE: Experimental and clinical studies support a role for the fetus in the control of the onset of labor. Fetal systemic inflammation, but not a maternal inflammatory response, has been linked to the onset of preterm labor and delivery on the basis of the determination of inflammatory cytokines in fetal and maternal blood. We propose that parturition requires fetomaternal cooperation and that inflammation is an integral part of the parturitional process. This study used flow cytometry, a sensitive technique for the detection of intravascular inflammation, to assess whether maternal inflammation is present in preterm labor. STUDY DESIGN: A prospective cross-sectional study was performed including patients with preterm labor (n = 55) and women with normal pregnancy (n = 50). Intravascular inflammation was studied by using flow cytometry. Maternal blood was assayed to determine granulocyte and monocyte phenotype by using monoclonal antibodies, which included the following cluster of differentiation (CD) markers: CD11b, CD14, CD15, CD16, CD18, CD49d, CD62L, CD64, CD66b, and HLA-DR. Oxidative burst and generation of basal intracellular oxygen radical species were assessed. Statistical analysis was conducted with the use of nonparametric methods. A P value of <.01 was considered statistically significant. RESULTS: Preterm labor was associated with a significant increase in the median mean channel brightness of CD11b, CD15, and CD66b on granulocytes and median mean channel brightness of CD11b and CD15 on monocytes. The ratio of oxidative burst over basal intracellular oxygen radical species in both granulocytes and monocytes was increased in preterm labor (P <.01). CONCLUSION: Preterm labor with intact membranes is associated with phenotypic and metabolic changes of maternal granulocytes and monocytes.

Original languageEnglish
Pages (from-to)1124-1129
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume185
Issue number5
DOIs
StatePublished - 1 Jan 2001

Fingerprint

Premature Obstetric Labor
Granulocytes
Monocytes
Mothers
Membranes
Inflammation
Respiratory Burst
Reactive Oxygen Species
Flow Cytometry
CD Antigens
Labor Onset
Endovascular Procedures
HLA-DR Antigens
Fetal Blood
Fetus
Cross-Sectional Studies
Monoclonal Antibodies
Parturition
Cytokines
Phenotype

Keywords

  • Inflammation
  • Leukocyte phenotype
  • Maternal inflammatory response
  • Parturition
  • Preterm labor

Cite this

Gervasi, Maria Teresa ; Chaiworapongsa, Tinnakorn ; Naccasha, Nihal ; Blackwell, Sean ; Yoon, Bohyun ; Maymon, Eli ; Romero, Roberto. / Phenotypic and metabolic characteristics of maternal monocytes and granulocytes in preterm labor with intact membranes. In: American Journal of Obstetrics and Gynecology. 2001 ; Vol. 185, No. 5. pp. 1124-1129.
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abstract = "OBJECTIVE: Experimental and clinical studies support a role for the fetus in the control of the onset of labor. Fetal systemic inflammation, but not a maternal inflammatory response, has been linked to the onset of preterm labor and delivery on the basis of the determination of inflammatory cytokines in fetal and maternal blood. We propose that parturition requires fetomaternal cooperation and that inflammation is an integral part of the parturitional process. This study used flow cytometry, a sensitive technique for the detection of intravascular inflammation, to assess whether maternal inflammation is present in preterm labor. STUDY DESIGN: A prospective cross-sectional study was performed including patients with preterm labor (n = 55) and women with normal pregnancy (n = 50). Intravascular inflammation was studied by using flow cytometry. Maternal blood was assayed to determine granulocyte and monocyte phenotype by using monoclonal antibodies, which included the following cluster of differentiation (CD) markers: CD11b, CD14, CD15, CD16, CD18, CD49d, CD62L, CD64, CD66b, and HLA-DR. Oxidative burst and generation of basal intracellular oxygen radical species were assessed. Statistical analysis was conducted with the use of nonparametric methods. A P value of <.01 was considered statistically significant. RESULTS: Preterm labor was associated with a significant increase in the median mean channel brightness of CD11b, CD15, and CD66b on granulocytes and median mean channel brightness of CD11b and CD15 on monocytes. The ratio of oxidative burst over basal intracellular oxygen radical species in both granulocytes and monocytes was increased in preterm labor (P <.01). CONCLUSION: Preterm labor with intact membranes is associated with phenotypic and metabolic changes of maternal granulocytes and monocytes.",
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Phenotypic and metabolic characteristics of maternal monocytes and granulocytes in preterm labor with intact membranes. / Gervasi, Maria Teresa; Chaiworapongsa, Tinnakorn; Naccasha, Nihal; Blackwell, Sean; Yoon, Bohyun; Maymon, Eli; Romero, Roberto.

In: American Journal of Obstetrics and Gynecology, Vol. 185, No. 5, 01.01.2001, p. 1124-1129.

Research output: Contribution to journalArticle

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T1 - Phenotypic and metabolic characteristics of maternal monocytes and granulocytes in preterm labor with intact membranes

AU - Gervasi, Maria Teresa

AU - Chaiworapongsa, Tinnakorn

AU - Naccasha, Nihal

AU - Blackwell, Sean

AU - Yoon, Bohyun

AU - Maymon, Eli

AU - Romero, Roberto

PY - 2001/1/1

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N2 - OBJECTIVE: Experimental and clinical studies support a role for the fetus in the control of the onset of labor. Fetal systemic inflammation, but not a maternal inflammatory response, has been linked to the onset of preterm labor and delivery on the basis of the determination of inflammatory cytokines in fetal and maternal blood. We propose that parturition requires fetomaternal cooperation and that inflammation is an integral part of the parturitional process. This study used flow cytometry, a sensitive technique for the detection of intravascular inflammation, to assess whether maternal inflammation is present in preterm labor. STUDY DESIGN: A prospective cross-sectional study was performed including patients with preterm labor (n = 55) and women with normal pregnancy (n = 50). Intravascular inflammation was studied by using flow cytometry. Maternal blood was assayed to determine granulocyte and monocyte phenotype by using monoclonal antibodies, which included the following cluster of differentiation (CD) markers: CD11b, CD14, CD15, CD16, CD18, CD49d, CD62L, CD64, CD66b, and HLA-DR. Oxidative burst and generation of basal intracellular oxygen radical species were assessed. Statistical analysis was conducted with the use of nonparametric methods. A P value of <.01 was considered statistically significant. RESULTS: Preterm labor was associated with a significant increase in the median mean channel brightness of CD11b, CD15, and CD66b on granulocytes and median mean channel brightness of CD11b and CD15 on monocytes. The ratio of oxidative burst over basal intracellular oxygen radical species in both granulocytes and monocytes was increased in preterm labor (P <.01). CONCLUSION: Preterm labor with intact membranes is associated with phenotypic and metabolic changes of maternal granulocytes and monocytes.

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