Performance of the QPLEX™ Alz plus assay, a novel multiplex kit for screening cerebral amyloid deposition

Jong Chan Park, Keum Sim Jung, Jiyeong Kim, Ji Sung Jang, Sunghoon Kwon, Min Soo Byun, Dahyun Yi, Gihwan Byeon, Gijung Jung, Yu Kyeong Kim, Dong Young Lee, Sun Ho Han, Inhee Mook-Jung

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Alzheimer’s disease (AD) is an irreversible neurodegenerative disease characterized by the hallmark finding of cerebral amyloid deposition. Many researchers have tried to predict the existence of cerebral amyloid deposition by using easily accessible blood plasma samples, but the effectiveness of such strategies remains controversial. Methods: We developed a new multiplex kit, the QPLEX™ Alz plus assay kit, which uses proteomics-based blood biomarkers to prescreen for cerebral amyloid deposition. A total of 300 participants who underwent Pittsburgh compound B (PiB)-positron emission tomography (PET) which allows imaging of cerebral amyloid deposition were included in this study. We compared the levels of QPLEX™ biomarkers between patients who were classified as PiB-negative or PiB-positive, regardless of their cognitive function. Logistic regression analysis followed by receiver operating characteristic (ROC) curve analysis was performed. The kit accuracy was tested using a randomized sample selection method. Results: The results obtained using our assay kit reached 89.1% area under curve (AUC) with 80.0% sensitivity and 83.0% specificity. Further validation of the QPLEX™ Alz plus assay kit using a randomized sample selection method showed an average accuracy of 81.5%. Conclusions: Our QPLEX™ Alz plus assay kit provides preliminary evidence that it can be used as blood marker to predict cerebral amyloid deposition but independent validation is needed.

Original languageEnglish
Article number12
JournalAlzheimer's Research and Therapy
Volume13
Issue number1
DOIs
StatePublished - Dec 2021

Keywords

  • Alzheimer’s disease
  • Blood-based biomarker
  • Cerebral amyloid deposition
  • Pittsburgh compound B
  • QPLEX™ Alz plus assay

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