Osteogenic differentiation of human bone marrow stromal cells cultured on hydroxyl carbonate apatite layer preformed on poly(ε-caprolactone)- organosiloxane nano-hybrid materials

Jeong Joon Yoo, Hee Joong Kim, Sang Hoon Rhee

Research output: Contribution to journalArticle

Abstract

Previous study showed that the novel poly(e-caprolactone)-organosiloxane nano-hybrid material (SiOPCL) had bioactivity, biodegradability, and mechanical properties comparable to those of human cancellous bone. In this study, hydroxyl carbonate apatite layer (HCA) was preformed on the surface of SiOPCL, which mimicked the events likely to occur in vivo, and cellular behaviors of human bone marrow stromal cells (hBMSCs) were investigated during the osteogenic differentiation on its surface (SiOPCL/HCA). Initial attachment, proliferation, and ALP activities of hBMSCs were comparable to those on tissue culture plates (TCPs), while the calcium content in the cell layer showed significantly higher value. It means that this novel bioactive nano-hybrid material is likely to be a promising candidate for bone grafting materials because of good hBMSCs responses as well as apatite forming ability in the simulated body fluid.

Original languageEnglish
Pages (from-to)573-576
Number of pages4
JournalKey Engineering Materials
Volume309-311 I
StatePublished - 6 Apr 2006

Fingerprint

Hybrid materials
Apatite
Hydroxyl Radical
Carbonates
Bone
Apatites
Tissue culture
Biodegradability
Body fluids
Bioactivity
Calcium
Cells
polycaprolactone
carboapatite
Mechanical properties

Keywords

  • Bone marrow stromal cell
  • Hydroxyl carbonate apatite
  • Osteogenic differentiation
  • Poly(e-caprolactone)
  • Siloxane

Cite this

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title = "Osteogenic differentiation of human bone marrow stromal cells cultured on hydroxyl carbonate apatite layer preformed on poly(ε-caprolactone)- organosiloxane nano-hybrid materials",
abstract = "Previous study showed that the novel poly(e-caprolactone)-organosiloxane nano-hybrid material (SiOPCL) had bioactivity, biodegradability, and mechanical properties comparable to those of human cancellous bone. In this study, hydroxyl carbonate apatite layer (HCA) was preformed on the surface of SiOPCL, which mimicked the events likely to occur in vivo, and cellular behaviors of human bone marrow stromal cells (hBMSCs) were investigated during the osteogenic differentiation on its surface (SiOPCL/HCA). Initial attachment, proliferation, and ALP activities of hBMSCs were comparable to those on tissue culture plates (TCPs), while the calcium content in the cell layer showed significantly higher value. It means that this novel bioactive nano-hybrid material is likely to be a promising candidate for bone grafting materials because of good hBMSCs responses as well as apatite forming ability in the simulated body fluid.",
keywords = "Bone marrow stromal cell, Hydroxyl carbonate apatite, Osteogenic differentiation, Poly(e-caprolactone), Siloxane",
author = "Yoo, {Jeong Joon} and Kim, {Hee Joong} and Rhee, {Sang Hoon}",
year = "2006",
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volume = "309-311 I",
pages = "573--576",
journal = "Key Engineering Materials",
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T1 - Osteogenic differentiation of human bone marrow stromal cells cultured on hydroxyl carbonate apatite layer preformed on poly(ε-caprolactone)- organosiloxane nano-hybrid materials

AU - Yoo, Jeong Joon

AU - Kim, Hee Joong

AU - Rhee, Sang Hoon

PY - 2006/4/6

Y1 - 2006/4/6

N2 - Previous study showed that the novel poly(e-caprolactone)-organosiloxane nano-hybrid material (SiOPCL) had bioactivity, biodegradability, and mechanical properties comparable to those of human cancellous bone. In this study, hydroxyl carbonate apatite layer (HCA) was preformed on the surface of SiOPCL, which mimicked the events likely to occur in vivo, and cellular behaviors of human bone marrow stromal cells (hBMSCs) were investigated during the osteogenic differentiation on its surface (SiOPCL/HCA). Initial attachment, proliferation, and ALP activities of hBMSCs were comparable to those on tissue culture plates (TCPs), while the calcium content in the cell layer showed significantly higher value. It means that this novel bioactive nano-hybrid material is likely to be a promising candidate for bone grafting materials because of good hBMSCs responses as well as apatite forming ability in the simulated body fluid.

AB - Previous study showed that the novel poly(e-caprolactone)-organosiloxane nano-hybrid material (SiOPCL) had bioactivity, biodegradability, and mechanical properties comparable to those of human cancellous bone. In this study, hydroxyl carbonate apatite layer (HCA) was preformed on the surface of SiOPCL, which mimicked the events likely to occur in vivo, and cellular behaviors of human bone marrow stromal cells (hBMSCs) were investigated during the osteogenic differentiation on its surface (SiOPCL/HCA). Initial attachment, proliferation, and ALP activities of hBMSCs were comparable to those on tissue culture plates (TCPs), while the calcium content in the cell layer showed significantly higher value. It means that this novel bioactive nano-hybrid material is likely to be a promising candidate for bone grafting materials because of good hBMSCs responses as well as apatite forming ability in the simulated body fluid.

KW - Bone marrow stromal cell

KW - Hydroxyl carbonate apatite

KW - Osteogenic differentiation

KW - Poly(e-caprolactone)

KW - Siloxane

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