Numerical and functional deficiencies of natural killer T cells in systemic lupus erythematosus: their deficiency related to disease activity.

Young Nan Cho, Seung Jung Kee, Sung Ji Lee, Seong Rye Seo, Tae Jong Kim, Shin Seok Lee, Min Soo Kim, Won Woo Lee, Dae Hyun Yoo, Nacksung Kim, Yong Wook Park

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78 Scopus citations

Abstract

This study was designed to examine the frequency of natural killer T (NKT) cells and the response to α-galactosylceramide (α-GalCer) in SLE patients and to investigate the clinical relevance of NKT cell levels. Patients with SLE (n = 128) and age- and sex-matched healthy controls (HCs) (n = 92) were enrolled in the study. NKT cell and CD1d levels were measured by flow cytometry. Gene expression was determined by RT-PCR, and cytokine secretion by multiple cytokine assay. Peripheral blood mononuclear cells (PBMCs) were cultured in vitro with α-GalCer. Proliferation indices of NKT cells were estimated by flow cytometry. Percentages and absolute numbers of NKT cells were significantly lower in the peripheral blood of SLE patients than in that of HCs, whereas CD1d levels in PBMCs were comparable between these two groups. Notably, this NKT cell deficiency was found to be correlated with SLEDAI. NKT cell proliferation was found to be impaired in SLE patients, and cytokine production by NKT cells in response to α-GalCer was diminished. This poor responsiveness to α-GalCer was found to be due to NKT cell dysfunction rather than to an abnormality in CD1d-expressing cells. Our data show that NKT cell levels and functions are defective in SLE patients. Furthermore, these deficiencies were found to reflect disease activity. It would appear that these NKT cell abnormalities could contribute to immune system dysregulation in SLE.

Original languageEnglish
Pages (from-to)1054-1063
Number of pages10
JournalRheumatology (Oxford, England)
Volume50
Issue number6
DOIs
StatePublished - Jun 2011

Bibliographical note

Funding Information:
Funding: This work was supported by grants from the Korea Research Foundation (KRF-2008-331-E00143), which was funded by the Korean Government (MOEHRD); the National Research Foundation of Korea Grant funded by the Korean Government (2009-0070590); the Chonnam National University Hospital Research Institute of Clinical Medicine (CRI10032-1); and the Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University.

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