NTA·Ni2+-functionalized quantum dots for VAMP2 labeling in live cells

Mi Kyung Yu, Suho Lee, Sunghoe Chang, Sangyong Jon

Research output: Contribution to journalArticle

Abstract

An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag (His6-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) (NTA·Ni2+) on the QD surfaces. We showed that the NTA·Ni2+-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted His 6-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.

Original languageEnglish
Pages (from-to)1474-1478
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume31
Issue number6
DOIs
StatePublished - 20 Jun 2010

Keywords

  • Imaging probes
  • Labeling
  • Protein tracking
  • Quantum dot conjugates

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