Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using 99m Tc-HMPAO

Do Won Hwang, Hongyoon Choi, Su Chul Jang, Min Young Yoo, Ji Yong Park, Na Eun Choi, Hyun Jeong Oh, Seunggyun Ha, Yun Sang Lee, Jae Min Jeong, Yong Song Gho, Dong Soo Lee

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances in biomedical applications of exosomes, it is crucial to understand in vivo distribution and behavior of exosomes. Here, we developed a simple method for radiolabeling of macrophage-derived exosome-mimetic nanovesicles (ENVs) with 99mTc-HMPAO under physiologic conditions and monitored in vivo distribution of 99mTc-HMPAO-ENVs using SPECT/CT in living mice. ENVs were produced from the mouse RAW264.7 macrophage cell line and labeled with 99mTc-HMPAO for 1 hr incubation, followed by removal of free 99mTc-HMPAO. SPECT/CT images were serially acquired after intravenous injection to BALB/c mouse. When ENVs were labeled with 99mTc-HMPAO, the radiochemical purity of 99mTc-HMPAO-ENVs was higher than 90% and the expression of exosome specific protein (CD63) did not change in 99mTc-HMPAO-ENVs. 99mTc-HMPAOENVs showed high serum stability (90%) which was similar to that in phosphate buffered saline until 5 hr. SPECT/CT images of the mice injected with 99mTc-HMPAO-ENVs exhibited higher uptake in liver and no uptake in brain, whereas mice injected with 99mTc-HMPAO showed high brain uptake until 5 hr. Our noninvasive imaging of radiolabeled-ENVs promises better understanding of the in vivo behavior of exosomes for upcoming biomedical application.

Original languageEnglish
Article number15636
JournalScientific Reports
Volume5
DOIs
StatePublished - 26 Oct 2015

Fingerprint

Technetium Tc 99m Exametazime
Exosomes
Macrophages
Brain
Intravenous Injections

Cite this

Hwang, Do Won ; Choi, Hongyoon ; Jang, Su Chul ; Yoo, Min Young ; Park, Ji Yong ; Choi, Na Eun ; Oh, Hyun Jeong ; Ha, Seunggyun ; Lee, Yun Sang ; Jeong, Jae Min ; Gho, Yong Song ; Lee, Dong Soo. / Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using 99m Tc-HMPAO. In: Scientific Reports. 2015 ; Vol. 5.
@article{8276e25ce44d4ef384aef975f39bea52,
title = "Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using 99m Tc-HMPAO",
abstract = "Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances in biomedical applications of exosomes, it is crucial to understand in vivo distribution and behavior of exosomes. Here, we developed a simple method for radiolabeling of macrophage-derived exosome-mimetic nanovesicles (ENVs) with 99mTc-HMPAO under physiologic conditions and monitored in vivo distribution of 99mTc-HMPAO-ENVs using SPECT/CT in living mice. ENVs were produced from the mouse RAW264.7 macrophage cell line and labeled with 99mTc-HMPAO for 1 hr incubation, followed by removal of free 99mTc-HMPAO. SPECT/CT images were serially acquired after intravenous injection to BALB/c mouse. When ENVs were labeled with 99mTc-HMPAO, the radiochemical purity of 99mTc-HMPAO-ENVs was higher than 90{\%} and the expression of exosome specific protein (CD63) did not change in 99mTc-HMPAO-ENVs. 99mTc-HMPAOENVs showed high serum stability (90{\%}) which was similar to that in phosphate buffered saline until 5 hr. SPECT/CT images of the mice injected with 99mTc-HMPAO-ENVs exhibited higher uptake in liver and no uptake in brain, whereas mice injected with 99mTc-HMPAO showed high brain uptake until 5 hr. Our noninvasive imaging of radiolabeled-ENVs promises better understanding of the in vivo behavior of exosomes for upcoming biomedical application.",
author = "Hwang, {Do Won} and Hongyoon Choi and Jang, {Su Chul} and Yoo, {Min Young} and Park, {Ji Yong} and Choi, {Na Eun} and Oh, {Hyun Jeong} and Seunggyun Ha and Lee, {Yun Sang} and Jeong, {Jae Min} and Gho, {Yong Song} and Lee, {Dong Soo}",
year = "2015",
month = "10",
day = "26",
doi = "10.1038/srep15636",
language = "English",
volume = "5",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using 99m Tc-HMPAO. / Hwang, Do Won; Choi, Hongyoon; Jang, Su Chul; Yoo, Min Young; Park, Ji Yong; Choi, Na Eun; Oh, Hyun Jeong; Ha, Seunggyun; Lee, Yun Sang; Jeong, Jae Min; Gho, Yong Song; Lee, Dong Soo.

In: Scientific Reports, Vol. 5, 15636, 26.10.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Noninvasive imaging of radiolabeled exosome-mimetic nanovesicle using 99m Tc-HMPAO

AU - Hwang, Do Won

AU - Choi, Hongyoon

AU - Jang, Su Chul

AU - Yoo, Min Young

AU - Park, Ji Yong

AU - Choi, Na Eun

AU - Oh, Hyun Jeong

AU - Ha, Seunggyun

AU - Lee, Yun Sang

AU - Jeong, Jae Min

AU - Gho, Yong Song

AU - Lee, Dong Soo

PY - 2015/10/26

Y1 - 2015/10/26

N2 - Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances in biomedical applications of exosomes, it is crucial to understand in vivo distribution and behavior of exosomes. Here, we developed a simple method for radiolabeling of macrophage-derived exosome-mimetic nanovesicles (ENVs) with 99mTc-HMPAO under physiologic conditions and monitored in vivo distribution of 99mTc-HMPAO-ENVs using SPECT/CT in living mice. ENVs were produced from the mouse RAW264.7 macrophage cell line and labeled with 99mTc-HMPAO for 1 hr incubation, followed by removal of free 99mTc-HMPAO. SPECT/CT images were serially acquired after intravenous injection to BALB/c mouse. When ENVs were labeled with 99mTc-HMPAO, the radiochemical purity of 99mTc-HMPAO-ENVs was higher than 90% and the expression of exosome specific protein (CD63) did not change in 99mTc-HMPAO-ENVs. 99mTc-HMPAOENVs showed high serum stability (90%) which was similar to that in phosphate buffered saline until 5 hr. SPECT/CT images of the mice injected with 99mTc-HMPAO-ENVs exhibited higher uptake in liver and no uptake in brain, whereas mice injected with 99mTc-HMPAO showed high brain uptake until 5 hr. Our noninvasive imaging of radiolabeled-ENVs promises better understanding of the in vivo behavior of exosomes for upcoming biomedical application.

AB - Exosomes known as nano-sized extracellular vesicles attracted recent interests due to their potential usefulness in drug delivery. Amid remarkable advances in biomedical applications of exosomes, it is crucial to understand in vivo distribution and behavior of exosomes. Here, we developed a simple method for radiolabeling of macrophage-derived exosome-mimetic nanovesicles (ENVs) with 99mTc-HMPAO under physiologic conditions and monitored in vivo distribution of 99mTc-HMPAO-ENVs using SPECT/CT in living mice. ENVs were produced from the mouse RAW264.7 macrophage cell line and labeled with 99mTc-HMPAO for 1 hr incubation, followed by removal of free 99mTc-HMPAO. SPECT/CT images were serially acquired after intravenous injection to BALB/c mouse. When ENVs were labeled with 99mTc-HMPAO, the radiochemical purity of 99mTc-HMPAO-ENVs was higher than 90% and the expression of exosome specific protein (CD63) did not change in 99mTc-HMPAO-ENVs. 99mTc-HMPAOENVs showed high serum stability (90%) which was similar to that in phosphate buffered saline until 5 hr. SPECT/CT images of the mice injected with 99mTc-HMPAO-ENVs exhibited higher uptake in liver and no uptake in brain, whereas mice injected with 99mTc-HMPAO showed high brain uptake until 5 hr. Our noninvasive imaging of radiolabeled-ENVs promises better understanding of the in vivo behavior of exosomes for upcoming biomedical application.

UR - http://www.scopus.com/inward/record.url?scp=84945282625&partnerID=8YFLogxK

U2 - 10.1038/srep15636

DO - 10.1038/srep15636

M3 - Article

C2 - 26497063

AN - SCOPUS:84945282625

VL - 5

JO - Scientific reports

JF - Scientific reports

SN - 2045-2322

M1 - 15636

ER -