Non-invasive in vivo imaging of caspase-1 activation enables rapid and spatiotemporal detection of acute and chronic inflammatory disorders

Young Ji Ko, Jae Won Lee, Eun Jeong Yang, Nayoon Jang, Jooho Park, Yoon Kyung Jeon, Je Wook Yu, Nam Hyuk Cho, Hye Sun Kim, Ick Chan Kwon

Research output: Contribution to journalArticle

Abstract

Inflammasome plays a critical role in diverse inflammatory disorders, including cancers and Alzheimer's disease. It is induced by various pathogenic insults and activates caspase-1, a hallmark executor of inflammasome. Here, we developed an activatable fluorescence probe for visualization of active caspase-1. This caspase-1 probe is biocompatible, efficiently delivered into cells and tissues, and specifically emits fluorescence upon caspase-1 activation as assessed in in vitro and in vivo models of inflammatory conditions. We demonstrated efficient in vivo imaging of caspase-1 activation in early stages of various inflammatory conditions of mice models, including endotoxin shock, inflammatory bowel disorder, transplanted cancer, and Alzheimer's disease. Notably, the caspase-1 probe enables detection of neuroinflammation in vivo two months earlier than cognitive impairments occur in Alzheimer's disease model. We detected significant fluorescence emitted from inflamed sites, as well as their draining lymph nodes, by macroscopic imaging analysis within 30 min after systemic injection of the probe. This novel synthetic probe could be applied for efficient and rapid detection of caspase-1 activity in a spatiotemporal way by non-invasive imaging.

Original languageEnglish
Article number119543
JournalBiomaterials
Volume226
DOIs
StatePublished - Jan 2020

Fingerprint

Caspase 1
Chemical activation
Imaging techniques
Fluorescence
Inflammasomes
Alzheimer Disease
Visualization
Tissue
Endotoxins
Shock
Neoplasms
Lymph Nodes
Injections

Keywords

  • Activatable fluorescence probe
  • Alzheimer's disease
  • Cancer
  • Caspase-1
  • Inflammasome
  • Inflammation

Cite this

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title = "Non-invasive in vivo imaging of caspase-1 activation enables rapid and spatiotemporal detection of acute and chronic inflammatory disorders",
abstract = "Inflammasome plays a critical role in diverse inflammatory disorders, including cancers and Alzheimer's disease. It is induced by various pathogenic insults and activates caspase-1, a hallmark executor of inflammasome. Here, we developed an activatable fluorescence probe for visualization of active caspase-1. This caspase-1 probe is biocompatible, efficiently delivered into cells and tissues, and specifically emits fluorescence upon caspase-1 activation as assessed in in vitro and in vivo models of inflammatory conditions. We demonstrated efficient in vivo imaging of caspase-1 activation in early stages of various inflammatory conditions of mice models, including endotoxin shock, inflammatory bowel disorder, transplanted cancer, and Alzheimer's disease. Notably, the caspase-1 probe enables detection of neuroinflammation in vivo two months earlier than cognitive impairments occur in Alzheimer's disease model. We detected significant fluorescence emitted from inflamed sites, as well as their draining lymph nodes, by macroscopic imaging analysis within 30 min after systemic injection of the probe. This novel synthetic probe could be applied for efficient and rapid detection of caspase-1 activity in a spatiotemporal way by non-invasive imaging.",
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Non-invasive in vivo imaging of caspase-1 activation enables rapid and spatiotemporal detection of acute and chronic inflammatory disorders. / Ko, Young Ji; Lee, Jae Won; Yang, Eun Jeong; Jang, Nayoon; Park, Jooho; Jeon, Yoon Kyung; Yu, Je Wook; Cho, Nam Hyuk; Kim, Hye Sun; Chan Kwon, Ick.

In: Biomaterials, Vol. 226, 119543, 01.2020.

Research output: Contribution to journalArticle

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AU - Park, Jooho

AU - Jeon, Yoon Kyung

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