Neurotensin enhances gastric motility in antral circular muscle strip of guinea-pig

Yong Koh Tae Yong Koh, Sungjoon Kim, Jin Lee Sang Jin Lee, Mook Kang Tong Mook Kang, Yeoul Jun Jae Yeoul Jun, Hoon Sim Jae Hoon Sim, Insuk So, Whan Kim Ki Whan Kim

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Many reports suggest that neurotensin (NT) in the gastrointestinal tract may play a possible role as a neurotransmitter, a circulating hormone, or a modulator of motor activity. NT exerts various actions in the intestine; it produces contractile and relaxant responses in intestinal smooth muscle. This study was designed to investigate the effect of NT on motility of antral circular muscle strips in guinea-pig stomach. To assess the role of Ca2+ influx in underlying mechanism, slow waves were simultaneously recorded with spontaneous contractions using conventional intracellular microelectrode technique. At the concentration of 10-7 M, where NT showed maximum response, NT enhanced the magnitude (863 ± 198%, mean ± SEM, n=13) and the frequency (154 ± 10.3%, n=11) of spontaneous contractions. NT evoked a slight hyperpolarization of membrane potential, tall and steep slow waves with abortive spikes (278 ± 50%, n=4). These effects were not affected by atropine (2 μM), guanethidine (2 μM) and tetrodotoxin (0.2 μM). NT-induced contractile responses were abolished in Ca2+-free solution and reduced greatly to near abolition by 10 μM of verapamil or 0.2 mM of CdCl2. Verapamil attenuated the effects of NT on frequency and amplitude of the slow waves. Taken together, these results indicate that NT enhances contractility in guinea-pig gastric antral circular muscle and Ca2+ influx through the voltage-operated Ca2+ channel appears to play an important role in the NT-induced contractile mechanism.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalKorean Journal of Physiology and Pharmacology
Volume4
Issue number3
StatePublished - 1 Jan 2000

Fingerprint

Neurotensin
Stomach
Guinea Pigs
Muscles
Verapamil
Antral
Guanethidine
Cadmium Chloride
Tetrodotoxin
Microelectrodes
Atropine
Membrane Potentials
Intestines
Smooth Muscle
Neurotransmitter Agents
Gastrointestinal Tract
Motor Activity
Hormones

Keywords

  • Ca influx
  • Neurotensin
  • Slow waves
  • Spontaneous contractions
  • Verapamil

Cite this

Tae Yong Koh, Y. K., Kim, S., Sang Jin Lee, J. L., Tong Mook Kang, M. K., Jae Yeoul Jun, Y. J., Jae Hoon Sim, H. S., ... Ki Whan Kim, W. K. (2000). Neurotensin enhances gastric motility in antral circular muscle strip of guinea-pig. Korean Journal of Physiology and Pharmacology, 4(3), 227-234.
Tae Yong Koh, Yong Koh ; Kim, Sungjoon ; Sang Jin Lee, Jin Lee ; Tong Mook Kang, Mook Kang ; Jae Yeoul Jun, Yeoul Jun ; Jae Hoon Sim, Hoon Sim ; So, Insuk ; Ki Whan Kim, Whan Kim. / Neurotensin enhances gastric motility in antral circular muscle strip of guinea-pig. In: Korean Journal of Physiology and Pharmacology. 2000 ; Vol. 4, No. 3. pp. 227-234.
@article{419ea0da94534e72aa3112be0b7c568e,
title = "Neurotensin enhances gastric motility in antral circular muscle strip of guinea-pig",
abstract = "Many reports suggest that neurotensin (NT) in the gastrointestinal tract may play a possible role as a neurotransmitter, a circulating hormone, or a modulator of motor activity. NT exerts various actions in the intestine; it produces contractile and relaxant responses in intestinal smooth muscle. This study was designed to investigate the effect of NT on motility of antral circular muscle strips in guinea-pig stomach. To assess the role of Ca2+ influx in underlying mechanism, slow waves were simultaneously recorded with spontaneous contractions using conventional intracellular microelectrode technique. At the concentration of 10-7 M, where NT showed maximum response, NT enhanced the magnitude (863 ± 198{\%}, mean ± SEM, n=13) and the frequency (154 ± 10.3{\%}, n=11) of spontaneous contractions. NT evoked a slight hyperpolarization of membrane potential, tall and steep slow waves with abortive spikes (278 ± 50{\%}, n=4). These effects were not affected by atropine (2 μM), guanethidine (2 μM) and tetrodotoxin (0.2 μM). NT-induced contractile responses were abolished in Ca2+-free solution and reduced greatly to near abolition by 10 μM of verapamil or 0.2 mM of CdCl2. Verapamil attenuated the effects of NT on frequency and amplitude of the slow waves. Taken together, these results indicate that NT enhances contractility in guinea-pig gastric antral circular muscle and Ca2+ influx through the voltage-operated Ca2+ channel appears to play an important role in the NT-induced contractile mechanism.",
keywords = "Ca influx, Neurotensin, Slow waves, Spontaneous contractions, Verapamil",
author = "{Tae Yong Koh}, {Yong Koh} and Sungjoon Kim and {Sang Jin Lee}, {Jin Lee} and {Tong Mook Kang}, {Mook Kang} and {Jae Yeoul Jun}, {Yeoul Jun} and {Jae Hoon Sim}, {Hoon Sim} and Insuk So and {Ki Whan Kim}, {Whan Kim}",
year = "2000",
month = "1",
day = "1",
language = "English",
volume = "4",
pages = "227--234",
journal = "Korean Journal of Physiology and Pharmacology",
issn = "1226-4512",
publisher = "Korean Physiological Soc. and Korean Soc. of Pharmacology",
number = "3",

}

Tae Yong Koh, YK, Kim, S, Sang Jin Lee, JL, Tong Mook Kang, MK, Jae Yeoul Jun, YJ, Jae Hoon Sim, HS, So, I & Ki Whan Kim, WK 2000, 'Neurotensin enhances gastric motility in antral circular muscle strip of guinea-pig', Korean Journal of Physiology and Pharmacology, vol. 4, no. 3, pp. 227-234.

Neurotensin enhances gastric motility in antral circular muscle strip of guinea-pig. / Tae Yong Koh, Yong Koh; Kim, Sungjoon; Sang Jin Lee, Jin Lee; Tong Mook Kang, Mook Kang; Jae Yeoul Jun, Yeoul Jun; Jae Hoon Sim, Hoon Sim; So, Insuk; Ki Whan Kim, Whan Kim.

In: Korean Journal of Physiology and Pharmacology, Vol. 4, No. 3, 01.01.2000, p. 227-234.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Neurotensin enhances gastric motility in antral circular muscle strip of guinea-pig

AU - Tae Yong Koh, Yong Koh

AU - Kim, Sungjoon

AU - Sang Jin Lee, Jin Lee

AU - Tong Mook Kang, Mook Kang

AU - Jae Yeoul Jun, Yeoul Jun

AU - Jae Hoon Sim, Hoon Sim

AU - So, Insuk

AU - Ki Whan Kim, Whan Kim

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Many reports suggest that neurotensin (NT) in the gastrointestinal tract may play a possible role as a neurotransmitter, a circulating hormone, or a modulator of motor activity. NT exerts various actions in the intestine; it produces contractile and relaxant responses in intestinal smooth muscle. This study was designed to investigate the effect of NT on motility of antral circular muscle strips in guinea-pig stomach. To assess the role of Ca2+ influx in underlying mechanism, slow waves were simultaneously recorded with spontaneous contractions using conventional intracellular microelectrode technique. At the concentration of 10-7 M, where NT showed maximum response, NT enhanced the magnitude (863 ± 198%, mean ± SEM, n=13) and the frequency (154 ± 10.3%, n=11) of spontaneous contractions. NT evoked a slight hyperpolarization of membrane potential, tall and steep slow waves with abortive spikes (278 ± 50%, n=4). These effects were not affected by atropine (2 μM), guanethidine (2 μM) and tetrodotoxin (0.2 μM). NT-induced contractile responses were abolished in Ca2+-free solution and reduced greatly to near abolition by 10 μM of verapamil or 0.2 mM of CdCl2. Verapamil attenuated the effects of NT on frequency and amplitude of the slow waves. Taken together, these results indicate that NT enhances contractility in guinea-pig gastric antral circular muscle and Ca2+ influx through the voltage-operated Ca2+ channel appears to play an important role in the NT-induced contractile mechanism.

AB - Many reports suggest that neurotensin (NT) in the gastrointestinal tract may play a possible role as a neurotransmitter, a circulating hormone, or a modulator of motor activity. NT exerts various actions in the intestine; it produces contractile and relaxant responses in intestinal smooth muscle. This study was designed to investigate the effect of NT on motility of antral circular muscle strips in guinea-pig stomach. To assess the role of Ca2+ influx in underlying mechanism, slow waves were simultaneously recorded with spontaneous contractions using conventional intracellular microelectrode technique. At the concentration of 10-7 M, where NT showed maximum response, NT enhanced the magnitude (863 ± 198%, mean ± SEM, n=13) and the frequency (154 ± 10.3%, n=11) of spontaneous contractions. NT evoked a slight hyperpolarization of membrane potential, tall and steep slow waves with abortive spikes (278 ± 50%, n=4). These effects were not affected by atropine (2 μM), guanethidine (2 μM) and tetrodotoxin (0.2 μM). NT-induced contractile responses were abolished in Ca2+-free solution and reduced greatly to near abolition by 10 μM of verapamil or 0.2 mM of CdCl2. Verapamil attenuated the effects of NT on frequency and amplitude of the slow waves. Taken together, these results indicate that NT enhances contractility in guinea-pig gastric antral circular muscle and Ca2+ influx through the voltage-operated Ca2+ channel appears to play an important role in the NT-induced contractile mechanism.

KW - Ca influx

KW - Neurotensin

KW - Slow waves

KW - Spontaneous contractions

KW - Verapamil

UR - http://www.scopus.com/inward/record.url?scp=0033858345&partnerID=8YFLogxK

M3 - Article

VL - 4

SP - 227

EP - 234

JO - Korean Journal of Physiology and Pharmacology

JF - Korean Journal of Physiology and Pharmacology

SN - 1226-4512

IS - 3

ER -

Tae Yong Koh YK, Kim S, Sang Jin Lee JL, Tong Mook Kang MK, Jae Yeoul Jun YJ, Jae Hoon Sim HS et al. Neurotensin enhances gastric motility in antral circular muscle strip of guinea-pig. Korean Journal of Physiology and Pharmacology. 2000 Jan 1;4(3):227-234.