Mucosal immune responses of mice experimentally infected with Pygidiopsis summa (Trematoda: Heterophyidae)

Jong Yil Chai, Young Jin Park, Jae Hwan Park, Bong Kwang Jung, Eun Hee Shin

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Mucosal immune responses against Pygidiopsis summa (Trematoda: Heterophyidae) infection were studied in ICR mice. Experimental groups consisted of group 1 (uninfected controls), group 2 (infection with 200 metacercariae), and group 3 (immunosuppression with Depo-Medrol and infection with 200 metacercariae). Worms were recovered in the small intestine at days 1, 3, 5, and 7 post-infection (PI). Intestinal intraepithelial lymphocytes (IEL), mast cells, and goblet cells were counted in intestinal tissue sections stained with Giemsa, astra-blue, and periodic acid-Schiff, respectively. Mucosal IgA levels were measured by ELISA. Expulsion of P. summa from the mouse intestine began to occur from days 3-5 PI which sustained until day 7 PI. The worm expulsion was positively correlated with proliferation of IEL, mast cells, goblet cells, and increase of IgA, although in the case of mast cells significant increase was seen only at day 7 PI. Immunosup-pression suppressed all these immune effectors and inhibited worm reduction in the intestine until day 7 PI. The results suggested that various immune effectors which include IEL, goblet cells, mast cells, and IgA play roles in regulating the intestinal mucosal immunity of ICR mice against P. summa infection.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalKorean Journal of Parasitology
Volume52
Issue number1
DOIs
StatePublished - 1 Jan 2014

Keywords

  • Goblet cell
  • IgA
  • Intestinal fluke
  • Intraepithelial lymphocyte
  • Mast cell
  • Mucosal immunity
  • Pygidiopsis summa

Cite this