Molecular tripods showing fluorescence enhancement upon binding to streptavidin

Tae Woo Kim, Young Yoon Hey, Jung Hyun Park, Oh Hoon Kwon, Du Jeon Jang, Jong In Hong

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

(Chemical Equation Presented) We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher-fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5-5.2 times that of molecular tripods in the absence of streptavidin.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalOrganic Letters
Volume7
Issue number1
DOIs
StatePublished - 6 Jan 2005

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tripods
Streptavidin
Fluorescence
Fluorophores
fluorescence
biotin
augmentation
Biotin
quenching
Ground state
Conformations
Quenching
Assays
ground state
interactions

Cite this

Kim, T. W., Hey, Y. Y., Park, J. H., Kwon, O. H., Jang, D. J., & Hong, J. I. (2005). Molecular tripods showing fluorescence enhancement upon binding to streptavidin. Organic Letters, 7(1), 111-114. https://doi.org/10.1021/ol047801h
Kim, Tae Woo ; Hey, Young Yoon ; Park, Jung Hyun ; Kwon, Oh Hoon ; Jang, Du Jeon ; Hong, Jong In. / Molecular tripods showing fluorescence enhancement upon binding to streptavidin. In: Organic Letters. 2005 ; Vol. 7, No. 1. pp. 111-114.
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Kim, TW, Hey, YY, Park, JH, Kwon, OH, Jang, DJ & Hong, JI 2005, 'Molecular tripods showing fluorescence enhancement upon binding to streptavidin', Organic Letters, vol. 7, no. 1, pp. 111-114. https://doi.org/10.1021/ol047801h

Molecular tripods showing fluorescence enhancement upon binding to streptavidin. / Kim, Tae Woo; Hey, Young Yoon; Park, Jung Hyun; Kwon, Oh Hoon; Jang, Du Jeon; Hong, Jong In.

In: Organic Letters, Vol. 7, No. 1, 06.01.2005, p. 111-114.

Research output: Contribution to journalArticle

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