Metallic Fe-Au Barcode Nanowires as a Simultaneous T Cell Capturing and Cytokine Sensing Platform for Immunoassay at the Single-Cell Level

Yoo Sang Jeon, Hyun Mu Shin, Yu Jin Kim, Da Yeon Nam, Bum Chul Park, Eunmin Yoo, Hang Rae Kim, Young Keun Kim

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Barcode nanowires (BNWs) composed of multiple layered segments of different materials are attractive to bioengineering field due to their characteristics that allow the adjustment of physicochemical properties and conjugation with two or more types of biomolecules to facilitate multiple tasks. Here, we report a metallic Fe (iron)-Au (gold) BNW-based platform for capturing CD8 T cells and the interferon-γ(γ) they secrete, both of which play key roles in controlling infectious diseases such as tuberculosis, at the single-cell level. We also describe an efficient approach for conjugating distinct antibodies, which recognize different epitopes to appropriate materials. The platform achieved detection even with 4.45-35.6 μg mL-1 of BNWs. The T cell capture efficiency was close to 100% and the detection limit for interferon-γwas 460 pg mL-1. This work presents a potential guideline for the design of single-cell immunoassay platforms for eliminating diagnostic errors by unambiguously identifying disease-relevant immune mediators.

Original languageEnglish
Pages (from-to)23901-23908
Number of pages8
JournalACS Applied Materials and Interfaces
Volume11
Issue number27
DOIs
StatePublished - 10 Jul 2019
Externally publishedYes

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Keywords

  • Fe-Au barcode nanowire
  • T cell capture
  • interferon-γmonitoring
  • single-cell immunoassay
  • tuberculosis

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