Menthol enhances an antiproliferative activity of 1α,25-dihydroxyvitamin D3 in LNCaP cells

Eun Jung Park, Su Hwa Kim, Byung Joo Kim, Sung Young Kim, Insuk So, Ju Hong Jeon

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3], the most active form of vitamin D3, and its analogues have therapeutic benefits for prostate cancer treatment. However, the development of hypercalcemia is an obstacle to clinical applications of 1α,25(OH)2D3 for cancer therapy. In this study, we provide evidence that menthol, a key component of peppermint oil, increases an anti-proliferation activity of 1α,25(OH)2D3 in LNCaP prostate cancer cells. We found that menthol per se does not exhibit antiproliferative activity, but it is able to enhance 1α,25(OH)2D3-mediated growth inhibition in LNCaP cells. Fluorometric assays using Fura-2 showed that 1α,25(OH)2D3 does not induce acute Ca2+ response, whereas menthol evokes an increase in [Ca2+]i, which suggests that cross-talks of menthol-induced Ca2+ signaling with 1α,25(OH)2D3-mediated growth inhibition pathways. In addition, Western blot analysis revealed that 1*25(OH)2D3 and menthol cooperatively modulate the expression of bcl-2 and p21 which provides the insight into the molecular mechanisms underlying the enhanced 1α,25(OH)2D3-mediated growth inhibition by menthol. Thus, our findings suggest that menthol may be a useful natural compound to enhance therapeutic effects of 1α,25(OH)2D3.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalJournal of Clinical Biochemistry and Nutrition
Volume44
Issue number2
DOIs
StatePublished - 1 Mar 2009

Fingerprint

Menthol
Calcitriol
Prostatic Neoplasms
Growth
Oncology
Fura-2
Cholecalciferol
Hypercalcemia
Therapeutic Uses
Assays
Western Blotting
Cells
Therapeutics

Keywords

  • 1α,25(OH)D
  • Anti-proliferation
  • Menthol
  • Prostate cancer

Cite this

Park, Eun Jung ; Kim, Su Hwa ; Kim, Byung Joo ; Kim, Sung Young ; So, Insuk ; Jeon, Ju Hong. / Menthol enhances an antiproliferative activity of 1α,25-dihydroxyvitamin D3 in LNCaP cells. In: Journal of Clinical Biochemistry and Nutrition. 2009 ; Vol. 44, No. 2. pp. 125-130.
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abstract = "1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3], the most active form of vitamin D3, and its analogues have therapeutic benefits for prostate cancer treatment. However, the development of hypercalcemia is an obstacle to clinical applications of 1α,25(OH)2D3 for cancer therapy. In this study, we provide evidence that menthol, a key component of peppermint oil, increases an anti-proliferation activity of 1α,25(OH)2D3 in LNCaP prostate cancer cells. We found that menthol per se does not exhibit antiproliferative activity, but it is able to enhance 1α,25(OH)2D3-mediated growth inhibition in LNCaP cells. Fluorometric assays using Fura-2 showed that 1α,25(OH)2D3 does not induce acute Ca2+ response, whereas menthol evokes an increase in [Ca2+]i, which suggests that cross-talks of menthol-induced Ca2+ signaling with 1α,25(OH)2D3-mediated growth inhibition pathways. In addition, Western blot analysis revealed that 1*25(OH)2D3 and menthol cooperatively modulate the expression of bcl-2 and p21 which provides the insight into the molecular mechanisms underlying the enhanced 1α,25(OH)2D3-mediated growth inhibition by menthol. Thus, our findings suggest that menthol may be a useful natural compound to enhance therapeutic effects of 1α,25(OH)2D3.",
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Menthol enhances an antiproliferative activity of 1α,25-dihydroxyvitamin D3 in LNCaP cells. / Park, Eun Jung; Kim, Su Hwa; Kim, Byung Joo; Kim, Sung Young; So, Insuk; Jeon, Ju Hong.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 44, No. 2, 01.03.2009, p. 125-130.

Research output: Contribution to journalArticle

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T1 - Menthol enhances an antiproliferative activity of 1α,25-dihydroxyvitamin D3 in LNCaP cells

AU - Park, Eun Jung

AU - Kim, Su Hwa

AU - Kim, Byung Joo

AU - Kim, Sung Young

AU - So, Insuk

AU - Jeon, Ju Hong

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N2 - 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3], the most active form of vitamin D3, and its analogues have therapeutic benefits for prostate cancer treatment. However, the development of hypercalcemia is an obstacle to clinical applications of 1α,25(OH)2D3 for cancer therapy. In this study, we provide evidence that menthol, a key component of peppermint oil, increases an anti-proliferation activity of 1α,25(OH)2D3 in LNCaP prostate cancer cells. We found that menthol per se does not exhibit antiproliferative activity, but it is able to enhance 1α,25(OH)2D3-mediated growth inhibition in LNCaP cells. Fluorometric assays using Fura-2 showed that 1α,25(OH)2D3 does not induce acute Ca2+ response, whereas menthol evokes an increase in [Ca2+]i, which suggests that cross-talks of menthol-induced Ca2+ signaling with 1α,25(OH)2D3-mediated growth inhibition pathways. In addition, Western blot analysis revealed that 1*25(OH)2D3 and menthol cooperatively modulate the expression of bcl-2 and p21 which provides the insight into the molecular mechanisms underlying the enhanced 1α,25(OH)2D3-mediated growth inhibition by menthol. Thus, our findings suggest that menthol may be a useful natural compound to enhance therapeutic effects of 1α,25(OH)2D3.

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