Magnetic implants in vivo guiding sorafenib liver delivery by superparamagnetic solid lipid nanoparticles

Rosa Maria Iacobazzi, Fabio Vischio, Ilaria Arduino, Fabio Canepa, Valentino Laquintana, Maria Notarnicola, Maria Principia Scavo, Giusy Bianco, Elisabetta Fanizza, Angela Assunta Lopedota, Annalisa Cutrignelli, Antonio Lopalco, Amalia Azzariti, Maria Lucia Curri, Massimo Franco, Gianluigi Giannelli, Byung Chul Lee, Nicoletta Depalo, Nunzio Denora

Research output: Contribution to journalArticlepeer-review

Abstract

Hypothesis: Solid lipid nanoparticles (SLNs), co-encapsulating superparamagnetic iron oxide nanoparticles and sorafenib, have been exploited for magnetic-guided drug delivery to the liver. Two different magnetic configurations, both comprising two small magnets, were under-skin implanted to investigate the effect of the magnetic field topology on the magnetic SLNP accumulation in liver tissues. A preliminary simulation analysis was performed to predict the magnetic field topography for each tested configuration. Experiments: SLNs were prepared using a hot homogenization approach and characterized using complementary techniques. Their in vitro biological behavior was assessed in HepG-2 liver cancer cells; wild-type mice were used for the in vivo study. The magnet configuration that resulted in a higher magnetic targeting efficiency was investigated by evaluating the iron content in homogenated murine liver tissues. Findings: SLNs, characterized by an average size smaller than 200 nm, retained their superparamagnetic behavior and relevant molecular resonance imaging properties as negative contrast agents. The evaluation of iron accumulation in the liver tissues was consistent with the magnetic induction profile of each magnet configuration, concurring with the results predicted by simulation analysis and obtained by measurements in living mice.

Original languageEnglish
Pages (from-to)239-254
Number of pages16
JournalJournal of Colloid and Interface Science
Volume608
DOIs
StatePublished - 15 Feb 2022

Keywords

  • HepG-2 Cancer Cells
  • Liver Magnetic Targeting
  • Magnetic Field Topography
  • SPIONs
  • Solid Lipid Nanoparticles
  • Sorafenib

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