Lung Matrix Metalloproteinase-9 Correlates with Cigarette Smoking and Obstruction of Airflow

Min Jong Kang, Yeon Mok Oh, Jae Cheol Lee, Dong Gyu Kim, Myung Jae Park, Myung Goo Lee, In Gyu Hyun, Sungkoo Han, Young Soo Shim, Ki Suck Jung

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Cigarette smoking is the most important risk factor for obstruction of airflow in chronic obstructive pulmonary disease (COPD). Matrix metalloproteinases (MMPs) or an imbalance between MMPs and their inhibitors, the tissue inhibitors of MMP (TIMPs), is considered to play a role in the pathogenesis of COPD. We investigated whether the MMPs expression or the imbalance between MMPs and TIMP-1 is associated with the amount of cigarette smoking and the FEV 1 value, in the lung parenchyma of 26 subjects (6 non-smokers and 20 cigarette smokers). First, we performed zymographic analysis to identify the profile of the MMPs, which revealed gelatinolytic bands mainly equivalent to MMP-9 in the smokers. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and its inhibitor, TIMP-1. Correlation analysis revealed that both the MMP-9 concentrations and the molar ratios of MMP-9 to TIMP-1 (MMP-9/TIMP-1) were correlated with the amount of cigarette smoking. Furthermore, MMP-9 concentrations were inversely correlated with FEV 1 . In conclusion, this study shows that MMP-9 expression in human lung parenchyma is associated with cigarette smoking and also with the obstruction of airflow, suggesting that MMP-9 may play a role in the pathogenesis of the cigarette smoke-induced obstruction of airflow known as the characteristic of COPD.

Original languageEnglish
Pages (from-to)821-827
Number of pages7
JournalJournal of Korean Medical Science
Volume18
Issue number6
DOIs
StatePublished - 1 Jan 2003

Fingerprint

Matrix Metalloproteinase 9
Smoking
Lung
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinases
Chronic Obstructive Pulmonary Disease
Tobacco Products
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase 1
Immunoenzyme Techniques
Smoke

Keywords

  • Chronic Obstructive
  • Lung Diseases
  • Matrix Metalloproteinases
  • Obstructive
  • Pulmonary Disease
  • Smoking
  • Tissue Inhibitor of Metalloproteinases

Cite this

Kang, M. J., Oh, Y. M., Lee, J. C., Kim, D. G., Park, M. J., Lee, M. G., ... Jung, K. S. (2003). Lung Matrix Metalloproteinase-9 Correlates with Cigarette Smoking and Obstruction of Airflow. Journal of Korean Medical Science, 18(6), 821-827. https://doi.org/10.3346/jkms.2003.18.6.821
Kang, Min Jong ; Oh, Yeon Mok ; Lee, Jae Cheol ; Kim, Dong Gyu ; Park, Myung Jae ; Lee, Myung Goo ; Hyun, In Gyu ; Han, Sungkoo ; Shim, Young Soo ; Jung, Ki Suck. / Lung Matrix Metalloproteinase-9 Correlates with Cigarette Smoking and Obstruction of Airflow. In: Journal of Korean Medical Science. 2003 ; Vol. 18, No. 6. pp. 821-827.
@article{c26d4b743510480cb8cf9ed508330851,
title = "Lung Matrix Metalloproteinase-9 Correlates with Cigarette Smoking and Obstruction of Airflow",
abstract = "Cigarette smoking is the most important risk factor for obstruction of airflow in chronic obstructive pulmonary disease (COPD). Matrix metalloproteinases (MMPs) or an imbalance between MMPs and their inhibitors, the tissue inhibitors of MMP (TIMPs), is considered to play a role in the pathogenesis of COPD. We investigated whether the MMPs expression or the imbalance between MMPs and TIMP-1 is associated with the amount of cigarette smoking and the FEV 1 value, in the lung parenchyma of 26 subjects (6 non-smokers and 20 cigarette smokers). First, we performed zymographic analysis to identify the profile of the MMPs, which revealed gelatinolytic bands mainly equivalent to MMP-9 in the smokers. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and its inhibitor, TIMP-1. Correlation analysis revealed that both the MMP-9 concentrations and the molar ratios of MMP-9 to TIMP-1 (MMP-9/TIMP-1) were correlated with the amount of cigarette smoking. Furthermore, MMP-9 concentrations were inversely correlated with FEV 1 . In conclusion, this study shows that MMP-9 expression in human lung parenchyma is associated with cigarette smoking and also with the obstruction of airflow, suggesting that MMP-9 may play a role in the pathogenesis of the cigarette smoke-induced obstruction of airflow known as the characteristic of COPD.",
keywords = "Chronic Obstructive, Lung Diseases, Matrix Metalloproteinases, Obstructive, Pulmonary Disease, Smoking, Tissue Inhibitor of Metalloproteinases",
author = "Kang, {Min Jong} and Oh, {Yeon Mok} and Lee, {Jae Cheol} and Kim, {Dong Gyu} and Park, {Myung Jae} and Lee, {Myung Goo} and Hyun, {In Gyu} and Sungkoo Han and Shim, {Young Soo} and Jung, {Ki Suck}",
year = "2003",
month = "1",
day = "1",
doi = "10.3346/jkms.2003.18.6.821",
language = "English",
volume = "18",
pages = "821--827",
journal = "Journal of Korean medical science",
issn = "1011-8934",
publisher = "Korean Academy of Medical Science",
number = "6",

}

Kang, MJ, Oh, YM, Lee, JC, Kim, DG, Park, MJ, Lee, MG, Hyun, IG, Han, S, Shim, YS & Jung, KS 2003, 'Lung Matrix Metalloproteinase-9 Correlates with Cigarette Smoking and Obstruction of Airflow', Journal of Korean Medical Science, vol. 18, no. 6, pp. 821-827. https://doi.org/10.3346/jkms.2003.18.6.821

Lung Matrix Metalloproteinase-9 Correlates with Cigarette Smoking and Obstruction of Airflow. / Kang, Min Jong; Oh, Yeon Mok; Lee, Jae Cheol; Kim, Dong Gyu; Park, Myung Jae; Lee, Myung Goo; Hyun, In Gyu; Han, Sungkoo; Shim, Young Soo; Jung, Ki Suck.

In: Journal of Korean Medical Science, Vol. 18, No. 6, 01.01.2003, p. 821-827.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Lung Matrix Metalloproteinase-9 Correlates with Cigarette Smoking and Obstruction of Airflow

AU - Kang, Min Jong

AU - Oh, Yeon Mok

AU - Lee, Jae Cheol

AU - Kim, Dong Gyu

AU - Park, Myung Jae

AU - Lee, Myung Goo

AU - Hyun, In Gyu

AU - Han, Sungkoo

AU - Shim, Young Soo

AU - Jung, Ki Suck

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Cigarette smoking is the most important risk factor for obstruction of airflow in chronic obstructive pulmonary disease (COPD). Matrix metalloproteinases (MMPs) or an imbalance between MMPs and their inhibitors, the tissue inhibitors of MMP (TIMPs), is considered to play a role in the pathogenesis of COPD. We investigated whether the MMPs expression or the imbalance between MMPs and TIMP-1 is associated with the amount of cigarette smoking and the FEV 1 value, in the lung parenchyma of 26 subjects (6 non-smokers and 20 cigarette smokers). First, we performed zymographic analysis to identify the profile of the MMPs, which revealed gelatinolytic bands mainly equivalent to MMP-9 in the smokers. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and its inhibitor, TIMP-1. Correlation analysis revealed that both the MMP-9 concentrations and the molar ratios of MMP-9 to TIMP-1 (MMP-9/TIMP-1) were correlated with the amount of cigarette smoking. Furthermore, MMP-9 concentrations were inversely correlated with FEV 1 . In conclusion, this study shows that MMP-9 expression in human lung parenchyma is associated with cigarette smoking and also with the obstruction of airflow, suggesting that MMP-9 may play a role in the pathogenesis of the cigarette smoke-induced obstruction of airflow known as the characteristic of COPD.

AB - Cigarette smoking is the most important risk factor for obstruction of airflow in chronic obstructive pulmonary disease (COPD). Matrix metalloproteinases (MMPs) or an imbalance between MMPs and their inhibitors, the tissue inhibitors of MMP (TIMPs), is considered to play a role in the pathogenesis of COPD. We investigated whether the MMPs expression or the imbalance between MMPs and TIMP-1 is associated with the amount of cigarette smoking and the FEV 1 value, in the lung parenchyma of 26 subjects (6 non-smokers and 20 cigarette smokers). First, we performed zymographic analysis to identify the profile of the MMPs, which revealed gelatinolytic bands mainly equivalent to MMP-9 in the smokers. We then measured, using enzyme immunoassay, the concentrations of MMP-9 and its inhibitor, TIMP-1. Correlation analysis revealed that both the MMP-9 concentrations and the molar ratios of MMP-9 to TIMP-1 (MMP-9/TIMP-1) were correlated with the amount of cigarette smoking. Furthermore, MMP-9 concentrations were inversely correlated with FEV 1 . In conclusion, this study shows that MMP-9 expression in human lung parenchyma is associated with cigarette smoking and also with the obstruction of airflow, suggesting that MMP-9 may play a role in the pathogenesis of the cigarette smoke-induced obstruction of airflow known as the characteristic of COPD.

KW - Chronic Obstructive

KW - Lung Diseases

KW - Matrix Metalloproteinases

KW - Obstructive

KW - Pulmonary Disease

KW - Smoking

KW - Tissue Inhibitor of Metalloproteinases

UR - http://www.scopus.com/inward/record.url?scp=0347694803&partnerID=8YFLogxK

U2 - 10.3346/jkms.2003.18.6.821

DO - 10.3346/jkms.2003.18.6.821

M3 - Article

C2 - 14676438

AN - SCOPUS:0347694803

VL - 18

SP - 821

EP - 827

JO - Journal of Korean medical science

JF - Journal of Korean medical science

SN - 1011-8934

IS - 6

ER -